Skip to main content
SpringerLink
Log in

Über die Virusempfindlichkeit von Schilddrüsen-Zellkulturen

  • Published:
Zeitschrift für Hygiene und Infektionskrankheiten, medizinische Mikrobiologie, Immunologie und Virologie Aims and scope Submit manuscript

Summary

Infected tissue culture fluid of prototype strains of various viruses was passed five times in human thyroid tissue cultures. Comparative titrations were carried out with both original culture fluid and virus from the fifth cell passage in thyroid cells as well as in HeLa or monkey kidney cells respectively. By this means thyroid cells were found to exhibit an equal (or in some instances higher) susceptibility as the culture of origin to vaccinia virus, herpes simplex virus, parainfluenza viruses types 1, 2 and 3, adenoviruses types 3, 6, 9 and 12, two prototype strains of Coxsackie B3 virus, Coxsackie A9 virus, ECHO 9 virus [prototype Hill and an epidemic strain (Russ.)], an attenuated and a virulent strain of each of the three poliovirus types. The virus yield in thyroid and the original cell system was found to be in the same range. ECHO 16 virus, two prototype strains of Coxsackie B4 virus and B.V.A. 96 strain of Coxsackie B2 virus produced no cytopathic effect in thyroid cell cultures and no inapparent multiplication was demonstrable. The Ohio 1 strain of Coxsackie B2 virus had a somewhat lower infectivity for thyroid than for monkey kidney cells. Reovirus type 1 showed minimal cytopathic change and only a slight multiplication in thyroid cells. From the ECHO virus group, the prototype strains of the types 1, 2, 3, 5, 6, 7, 8, 11, 12, 13, 19, 20, 22 and 24 could be passed readily with CPE in thyroid cells, whereas ECHO types 18 and 23 were erratic and type 17 produced no CPE. The prototype strains of adenovirus types 1 to 28 as well as 24 other strains of various serotypes were cytopathic for thyroid cells without exception. Our experience with the culture and maintenance of human thyroid cells as obtained in the course of this study has been reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Ababio, A. R.: Viral susceptibility of human thyroid tissue culture. Doktor-Dissertation. Homburg (Saar) 1962.

  2. Archetti, I., J. Weston andH. A. Wenner: Adaptation of ECHO viruses in HeLa cells; their use in complement fixation. Proc. Soc. exp. Biol. (N. Y.)95, 265–270 (1957).

    Google Scholar 

  3. Crowell, R. L., andJ. T. Syverton: The viral range in vitro of a malignant, human epithelial cell (strain HeLa, Gey). IV. The cytopathogenicity of C viruses. J. Immunol.74, 169–177 (1955).

    PubMed  Google Scholar 

  4. Duncan, I. B. R.: Human thyroid tissue culture in diagnostic virology. Arch. ges. Virusforsch.10, 490–500 (1960).

    Google Scholar 

  5. —, andJ. G. P. Hutchinson: Type-3 adenovirus infection with gastrointestinal symptoms. Lancet1961 I, 530–532.

    Google Scholar 

  6. Eggers, H. J., andI. Tamm: 2(α-hydroxybenzyl)-benzimidazole (HBB) as an aid in virus classification. Virology13, 545–546 (1961).

    PubMed  Google Scholar 

  7. Hövel, H., u.W. Schneider: Struma in der Gewebekultur und ihre Eignung für Arbeiten mit dem Poliomyelitis-Virus. Zbl. Bakt., I. Abt. Orig.177, 285–288 (1960).

    Google Scholar 

  8. Lehmann-Grube, F.: Comparative susceptibility of mammalian cells in culture to prototype enteroviruses. Arch. ges. Virusforsch.11, 276–283 (1961).

    PubMed  Google Scholar 

  9. Pulvertaft, R. J. V., J. R. Davies, L. Weiss andJ. H. Wilkinson: Studies on tissue cultures of human pathological thyroids. J. Path. Bact.77, 19–32 (1959).

    PubMed  Google Scholar 

  10. Reed, L. J., andH. Muench: A simple method of estimating fifty per cent endpoints. Amer. J. Hyg.27, 493–497 (1938).

    Google Scholar 

  11. Schneider, W., H.Hövel u. J.Krüger: Das Verhalten von Poliomyelitis. Virus auf Strumagewebekulturen. Arb. Paul-Ehrlich-Institut Heft 56, 55–61 (1961).

  12. Shaver, D. N., A. L. Barron andD. T. Karzon: Distinctive cytopathology of ECHO viruses types 22 and 23. Proc. Soc. exp. Biol. (N. Y.)106, 648–652 (1961).

    Google Scholar 

  13. Vogel, J., andA. Shelokov: Adsorption-hemagglutination test for influenza virus in monkey kidney tissue culture. Science126, 358–359 (1957).

    PubMed  Google Scholar 

  14. Wigand, R., u.A. B. Sabin: Properties of ECHO types 22, 23 and 24 viruses. Arch. ges. Virusforsch.11, 224–247 (1961).

    PubMed  Google Scholar 

  15. — —: Intratypic antigenic heterogeneity of Coxsackie B viruses. Arch. ges. Virusforsch.12, 29–41 (1962).

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. A. R. Ababio

    Present address: Alexander von Humboldt-Stipendiat an der Universitäts-Kinderklinik, Homburg

Authors and Affiliations

  1. Institut für Hygiene und Mikrobiologie der Universität des Saarlandes Homburg (Saar), Deutschland

    A. R. Ababio, H. Bauer & R. Wigand

Authors
  1. A. R. Ababio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Bauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Wigand
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Die vorliegenden Untersuchungen wurden mit Unterstützung der Deutschen Vereinigung zur Bekämpfung der Kinderlähmung e. V. und der Deutschen Forschungsgemeinschaft durchgeführt. Einer von uns (A. R. A.) verdankt dem Ghana Cocoa Marketing Board ein Stipendium. Die Schilddrüsen erhielten wir durch die Freundlichkeit von Herrn Prof. Dr.Lüdeke, Chirurgische Universitätsklinik, Homburg, von Herrn Chefarzt Dr.Schäfer, Kreiskrankenhaus St. Ingbert, und von Herrn Oberarzt Dr.Schrauf, Bürgerhospital Saarbrücken. Ganz besonders danken wir Fräulein E.Baschleben, Fräulein I.Lamy und Frau H.Huwer für ihre technische Hilfe bei den Untersuchungen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ababio, A.R., Bauer, H. & Wigand, R. Über die Virusempfindlichkeit von Schilddrüsen-Zellkulturen. Zeitschr. f. Hygiene. 149, 82–95 (1963). https://doi.org/10.1007/BF02157508

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02157508

Search

Navigation