Mycopathologia et mycologia applicata

, Volume 13, Issue 2, pp 113–120 | Cite as

Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol

  • E. S. Mc Donough
  • Lucille K. Georg
  • Libero Ajello
  • Sherry Brinkman


Studies of 24 strains ofBlastomyces dermatitidis confirmed previously published results that the yeast-phase of this fungus is more sensitive than the mycelial-phase to cycloheximide and chloramphenicol.

Studies of 5 strains each ofHistoplasma capsulatum, Paracoccidioides brasiliensis andSporotrichum schenckii show that that these species also have a similar yeast-phase mycelial -phase sensitivity differential in regard to these antibiotics.

A cycloheximide resistant strain ofB. dermatitidis was developed from a sensitive strain.

The experimental results support the general practice of using 0.5 mg/ml cycloheximide and 0.05 mg/ml chloramphenicol in media for the isolation of the four fungi at 25° C. The results indicate, however, that some strains would not be recovered at 37° C with similar concentrations of these antibiotics.

It is recommended that a concentration of not more than 0.2 mg/ml chloramphenicol should be used to preserve sputum which is subsequently to be cultured forB. dermatitidis, Histoplasma capsulatum, Paracoccidioides brasiliensis orS. schenckii.


General Practice Chloramphenicol Resistant Strain Cycloheximide Similar Concentration 
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Copyright information

© Dr. W. Junk 1960

Authors and Affiliations

  • E. S. Mc Donough
  • Lucille K. Georg
  • Libero Ajello
  • Sherry Brinkman
    • 1
  1. 1.From the Mycology Unit, Communicable Disease CenterU.S. Public Health Service, Department of Health, Education and WelfareAtlanta

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