Advertisement

Mycopathologia et mycologia applicata

, Volume 13, Issue 2, pp 113–120 | Cite as

Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol

  • E. S. Mc Donough
  • Lucille K. Georg
  • Libero Ajello
  • Sherry Brinkman
Article

Summary

Studies of 24 strains ofBlastomyces dermatitidis confirmed previously published results that the yeast-phase of this fungus is more sensitive than the mycelial-phase to cycloheximide and chloramphenicol.

Studies of 5 strains each ofHistoplasma capsulatum, Paracoccidioides brasiliensis andSporotrichum schenckii show that that these species also have a similar yeast-phase mycelial -phase sensitivity differential in regard to these antibiotics.

A cycloheximide resistant strain ofB. dermatitidis was developed from a sensitive strain.

The experimental results support the general practice of using 0.5 mg/ml cycloheximide and 0.05 mg/ml chloramphenicol in media for the isolation of the four fungi at 25° C. The results indicate, however, that some strains would not be recovered at 37° C with similar concentrations of these antibiotics.

It is recommended that a concentration of not more than 0.2 mg/ml chloramphenicol should be used to preserve sputum which is subsequently to be cultured forB. dermatitidis, Histoplasma capsulatum, Paracoccidioides brasiliensis orS. schenckii.

Keywords

General Practice Chloramphenicol Resistant Strain Cycloheximide Similar Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    McDonough, E. S., Ajello, L., Georg, L. K. &Brinkman, S. (1960) In vitro effects of antibiotics on the yeast phase of Blastomyces dermatitidis and other fungi, J. Lab. & Clin. Med50 116–119.Google Scholar
  2. 2.
    Fuentes, C. A., Trespalacios, F., Baquero, G. F. &Aboulafia, R. (1952) Effects of acti-dione on mold contaminants and on human pathogens, Mycologia44 170–175.Google Scholar
  3. 3.
    Georg, L. K. (1953) Use of a cycloheximide medium for isolation of dermatophytes from clinical materials, Arch. Derm. Syph.67 355–361.Google Scholar
  4. 4.
    Ajello, L. &Getz, M. E. (1954) Recovery of dermatophytes from shoes and shower stalls, J. Invest. Derm.22 17–21.PubMedGoogle Scholar
  5. 5.
    Sharvill, D. &Talbot, J. M. (1954) Cycloheximide in the isolation of dermatophytes, Brit. J. Derm.66 214–217.PubMedGoogle Scholar
  6. 6.
    Fegeler, F. (1956) Untersuchungen zur Epidemiologie der Epidermophytien mit besonderer Berücksichtigung desTrichophyton rubrum (Castellani), Arch. klin. exp. Derm.203 570–576.CrossRefPubMedGoogle Scholar
  7. 7.
    Rosenthal, S. A., Baer, R. L., Litt, L. Z., Rogachefsky, H. &Furnari, D. (1956) Studies on the dissemination of fungi from the feet of subjects with and without diseases of the feet, J. Invest. Dermat.26 41–47.Google Scholar
  8. 8.
    Rosenthal, S. A. &Furnari, D. (1957) The use of cycloheximide-chloramphenicol medium in routine culture for fungi, J. Invest. Derm.28 367–371.PubMedGoogle Scholar
  9. 9.
    Adam, W. &Steitz, K. (1958) Zur Wirkung von Cycloheximid auf Pilzkulturen, Z. Haut-u. Geschlechtskr.25 153–161.Google Scholar
  10. 10.
    Coudert, J. &Murat, M. (1954) Intéret du milieu de Sabouraud à la chloramycétine pour l'isolement des dermatophytes et des champignons levuriformes. Ann. Biol Clin.12(3–4), 185–186.Google Scholar

Copyright information

© Dr. W. Junk 1960

Authors and Affiliations

  • E. S. Mc Donough
  • Lucille K. Georg
  • Libero Ajello
  • Sherry Brinkman
    • 1
  1. 1.From the Mycology Unit, Communicable Disease CenterU.S. Public Health Service, Department of Health, Education and WelfareAtlanta

Personalised recommendations