Abstract
We injected varying numbers of retinal Muller glia into the rabbit vitreous in an established model of traction retinal detachment. We used indirect ophthalmoscopy to observe the changes elicited during the following 1 month. Although the severity of the tractional changes increased with increasing numbers of the glial cells, the pathology produced stabilized within the 1st week of injury. Muller glia were less effective at eliciting retinal detachments than retinal pigment epithelial cells (RPE) or mixtures of glia and RPE. Intravitreal tissue membranes derived from the glia differed morphologically from those derived from RPE. The glial membranes had fewer fibroblast-like cells, synthesized less extracellular matrix, and showed lower intravitreal cell proliferation, as determined by3H-thymidine radioautography. Our findings indicate that membranes composed only of Muller glial cells promote less severe retinal pathology than those membranes composed of RPE or mixed cell types.
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Algvere P, Kock E (1976) Experimental fibroplasia in the rabbit vitreous. Effects of hyaluronidase and implantation of autologous dermal tissue. Graefe's Arch Clin Exp Ophthalmol 199: 133–139
Burke JM (1982) Cultured retinal glial cells are insensitive to platelet-derived growth factor. Exp Eye Res 35: 663–669
Burke JM (1983) Cell-cell contact promotes DNA synthesis in retinal glia but not in fibroblasts. Exp Cell Res 146: 204–206
Burke JM, Foster SJ (1982) Injured vitreous stimulates DNA synthesis in retinal pigment epithelial cells in culture and within the vitreous. Graefe's Arch Clin Exp Ophthalmol 218: 153–155
Burke JM, Foster SJ (1984) Culture of rabbit retinal glial cells: methods and cellular origin of explant outgrowth. Curr Eye Res 3: 1169–1178
Burke JM, Foster SJ (1985a) Corneal stromal fibroblasts from adult rabbits retain the capacity to deposit an orthogonal matrix. Dev Biol 108: 250–253
Burke JM, Foster SJ (1985b) Induction of DNA synthesis by co-culture of retinal glia and pigment epithelium. Invest Ophthalmol Vis Sci 26: 636–642
Burke JM, Kower HS (1980) Collagen synthesis by rabbit neural retinain vitro andin vivo. Exp Eye Res 31: 213–226
Fastenberg DM, Diddie KR, Sorgente N, Ryan SJ (1982a) A comparison of different cellular inocula in an experimental model of massive periretinal proliferation. Am J Ophthalmol 93: 559–564
Fastenberg DM, Diddie KR, Dorey K, Ryan SJ (1982b) The role of cellular proliferation in an experimental model of massive periretinal proliferation. Am J Ophthalmol 93: 565–572
Hiscott PS, Grierson I, Trombetta CJ (1984a) Retinal glia and epiretinal glia — an immunohistochemical study. Br J Ophthalmol 68: 698–707
Hiscott PS, Grierson I, McLeod D (1984b) Retinal pigment epithelial cells in epiretinal membranes: an immunohistochemical study. Br J Ophthalmol 68: 708–715
Kampik A, Kenyon K, Michels R, Green WR, de la Cruz Z (1981) Epithelial and vitreous membranes. Comparative study of 56 cases. Arch Ophthalmol 99: 1445–1454
Machemer R, Laqua H (1975) Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80: 1–23
Radtke N, Tano Y, Chandler DB, Machemer R (1981) Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits. Am J Ophthalmol 91: 76–87
Rodrigues MM, Newsome D, Machemer R (1981) Further characterization of epiretinal membranes in human massive periretinal proliferation. Curr Eye Res 1: 311–315
Shaw G, Weber K (1984) The intermediate filament complement of the retina: a comparison between different mammalian species. Eur J Cell Biol 33: 95–104
Sugita G, Tano Y, Machemer R, Abrams G, Claflin A, Fiorentino G (1980) Intravitreal autotransplantation of fibroblasts. Am J Ophthalmol 89: 121–130
Tano Y, Chandler DB, Machemer R (1981) Retinal neovascularization after intravitreal fibroblast injection. Am J Ophthalmol ???92-103–109
Tomasek JJ, Hay ED (1984) Analysis of the role of microfilaments and microtubules in acquisition of bipolarity and elongation of fibroblasts in hydrated collagen gels. J Cell Biol 99: 536–549
Tripathi B, Ashton N (1971) Vaso-glial connections in the rabbit retina. Br J Ophthalmol 55: 1–11
Ussmann HH, Lazarides E, Ryan SJ (1981) Traction retinal detachment. A cell mediated event. Arch Ophthalmol 99: 869–872
Van Horn DL, Aaberg TM, Machemer R, Fenzl R (1977) Glial cell proliferation in human retinal detachment with massive periretinal proliferation. Am J Ophthalmol 84: 383–393
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This study was supported in part by National Eye Institute grant EY04799 (J.M.B.), Core Center Grant EY01931 (J.M.B.), an unrestricted grant from Research to Prevent Blindness, Inc., and the Good Samaritan Foundation for Ophthalmic Research (Portland, Oregon)
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Peters, M.A., Burke, J.M., Clowry, M. et al. Development of traction retinal detachments following intravitreal injections of retinal Muller and pigment epithelial cells. Graefe's Arch Clin Exp Ophthalmol 224, 554–563 (1986). https://doi.org/10.1007/BF02154745
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DOI: https://doi.org/10.1007/BF02154745