Skip to main content
SpringerLink
Log in

The effect of topical cyclosporin A on the rabbit cornea

A clinical and electron microscopic study

  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

To investigate the effect of topical cyclosporin A (CSA) on the rabbit cornea, both eyes of 80 animals were treated with CSA 2% eye drops over a 3-week period under various conditions. CSA was dissolved in castor oil or ethanol 13.8 vol. %. Slit-lamp, scanning and transmission electron microscopic examinations were performed. CSA 2% in castor oil given 5 times daily showed no damage to either the corneal epithelium or endothelium. In contrast the solutions containing ethanol revealed considerable epithelial cell damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahonen J, Nemlander A, Wiktorowicz K, von Willebrand E, Hekali R, Lalla M, Häyry P (1984) Effect of cyclosporine on wound healing. In: Kahan BD (ed) Cyclosporine. Biological activity and clinical applications. Grune & Stratton, Orlando, pp 876–877

    Google Scholar 

  • Behrens-Baumann W, Theuring S, Frey B, Frey F, Sostmann H, Bircher J (1986) Cyclosporine concentrations in the rabbit aqueous humor and cornea following subconjunctival administration. Importance of anatomical site of injection. Graefe's Arch Clin Exp Ophthalmol 224: 368–370

    Article  Google Scholar 

  • Bell TAG, Easty DL, McCullagh KG (1982) A placebo-controlled blind trial of cyclosporin A in prevention of corneal graft rejection in rabbits. Br J Ophthalmol 66: 303–308

    PubMed  Google Scholar 

  • Brewitt H, Feuerhake H (1980) Der Einfluß von Desinfektionslösungen für HEMA-Kontaktlinsen auf das Hornhautepithel — eine rasterelektronenmikroskopische Untersuchung. Contactologia 2: 262–272

    Google Scholar 

  • Brewitt H, Honegger H (1979) Early morphological changes of the corneal epithelium after burning with hydrochloric acid. A scanning electron microscope study. Ophthalmologica (Basel) 178: 327–336

    Google Scholar 

  • Burstein NL (1980) Preservative cytotoxic threshold for benzalkonium chloride and chlorhexidine digluconate in cat and rabbit corneas. Invest Ophthalmol Vis Sci 19: 308–313

    PubMed  Google Scholar 

  • Coster DJ, Shepherd WFI, Chin Fook T, Rice NSC, Jones BR (1979) Prolonged survival of corneal allografts in rabbits treated with cyclosporin A. Lancet II: 688–689

    Article  Google Scholar 

  • Editorial (1979) Cyclosporin A. Lancet II : 779–780

  • Grant WM (1974) Toxicology of the eye. Charles C. Thomas, Springfield, pp 467–472

    Google Scholar 

  • Hunter PA (1981) Potential role of cyclosporin A in corneal grafting. J Roy Soc Med 74: 810–813

    Google Scholar 

  • Hunter PA, Wilhelmus KR, Rice NSC, Jones BR (1981) Cyclosporin A applied topically to the recipient eye inhibits corneal graft rejection. Clin Exp Immunol 45: 173–177

    PubMed  Google Scholar 

  • Hunter PA, Rice NSC, Jones BR (1982a) Prolonged corneal graft survival using topically applied cyclosporin A. Trans Ophthal Soc UK 102: 19–20

    Google Scholar 

  • Hunter PA, Garner A, Wilhelmus KR, Rice NSC, Jones BR (1982b) Corneal graft injection: a new rabbit model and cyclosporin A. Br J Ophthalmol 66: 292–302

    Google Scholar 

  • Kana JS, Hoffmann F, Buchen R, Krolik A, Wiederholt M (1982) Rabbit corneal allograft survival following topical administration of cyclosporin A. Invest Ophthalmol Vis Sci 22: 686–690

    PubMed  Google Scholar 

  • Mosteller MV, Gebhardt BM, Hamilton AM, Kaufman HE (1985) Penetration of topical cyclosporine into the rabbit cornea, aqueous humor and serum. Arch Ophthalmol 103: 101–102

    PubMed  Google Scholar 

  • Pfister RR, Burstein N (1976) The effects of ophthalmic drugs, vehicles, and preservatives on corneal epithelium: a scanning electron microscope study. Invest Ophthalmol Vis Sci 15: 246–259

    Google Scholar 

  • Salisbury JD, Gebhardt BM (1981) Suppression of corneal allograft rejection by cyclosporin A. Arch Ophthalmol 99: 1640–1643

    PubMed  Google Scholar 

  • Shepherd WFI, Coster DJ, Chin Fook T, Rice NSC, Jones BR (1980) Effect of cyclosporin A on the survival of corneal grafts in rabbits. Br J Ophthalmol 64: 148–153

    PubMed  Google Scholar 

  • Tønjum AM (1975) Effects of benzalkonium chloride upon the corneal epithelium studied with scanning electron microscopy. Acta Ophthalmol (Copenh) 53: 358–366

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, University of Göttingen, Federal Republic of Germany

    W. Behrens-Baumann & S. Theuring

  2. Department of Opthalmology, University of Hannover, Federal Republic of Germany

    H. Brewitt

Authors
  1. W. Behrens-Baumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Theuring
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Brewitt
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Presented at the meeting of the Northwest German Society of Ophthalmology (Nordwestdeutsche Augenarzte), Hamburg, FRG, 2 June, 1985

Rights and permissions

Reprints and permissions

About this article

Cite this article

Behrens-Baumann, W., Theuring, S. & Brewitt, H. The effect of topical cyclosporin A on the rabbit cornea. Graefe's Arch Clin Exp Ophthalmol 224, 520–524 (1986). https://doi.org/10.1007/BF02154739

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02154739

Keywords

Search

Navigation