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A histochemical method for the demonstration of fat peroxides

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Zusammenfassung

Es wird ein histochemisches Verfahren zum Nachweis von Fettperoxyden beschrieben. Als Objekte dienen einerseits gefrorene Fettgewebsschnitte von Ratten, die eine vitamin-E-freie und an Dorschlebertran reiche Kost erhalten hatten, und andererseits entsprechende Präparate von Kontrolltieren. Die Methode basiert auf der Oxydation der Leukobase des 2,6-Dichlorphenolindophenols durch Zellperoxyde. Die Reaktion wird durch Hämin in Gegenwart von Essigsäure und Alkohol katalysiert. Die rote Farbe des bei der Reaktion gebildeten Indophenols erscheint in allen denjenigen Zellen und Zellstrukturen, in denen Peroxyde vorhanden sind. Das Verfahren dürfte in abgeänderter Form auch für den Nachweis von Peroxyden in anderen Geweben verwendbar sein.

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References

  1. H. Dam, J. Nutrition27, 193 (1944);28, 297 (1944).

    Article  CAS  Google Scholar 

  2. H. Granados andH. Dam, Science101, 250 (1945); Proc. Soc. Exp. Biol. Med.59, 295 (1945).

    Article  CAS  PubMed  Google Scholar 

  3. H. Dam andH. Granados, Acta Physiol. Scand.10, 162 (1945).

    Article  CAS  Google Scholar 

  4. H. Dam andH. Granados, Science102, 327 (1945).

    Article  CAS  PubMed  Google Scholar 

  5. P. Gyorgy andH. Goldblatt, J. Exp. Med.75, 355 (1942).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. K. M. Endicott andR. D. Lillie, Amer. J. Pathol.20, 149 (1944).

    CAS  Google Scholar 

  7. H. Dam andK. E. Mason, Fed. Proc.4, 153 (1945).

    Google Scholar 

  8. K. E. Mason, H. Dam, andH. Granados, Anat. Rec.94, 265 (1946).

    Article  CAS  PubMed  Google Scholar 

  9. H. Granados, K. E. Mason, andH. Dam, Acta Pathol. et Microbiol. Scand.24, 86 (1947).

    Article  CAS  Google Scholar 

  10. H. Dam, J. Nutrition28, 297 (1944).

    Article  CAS  Google Scholar 

  11. H. Dam andH. Granados, Acta Physiol. Scand.10, 162 (1945).

    Article  CAS  Google Scholar 

  12. K. E. Mason, H. Dam, andH. Granados, Anat. Rec.94, 265 (1946).

    Article  CAS  PubMed  Google Scholar 

  13. H. Dam andH. Granados, Science102, 327 (1945).

    Article  CAS  PubMed  Google Scholar 

  14. J. Glavind andS. Hartmann, Acta Physiol. Scand.16, suppl. 53, 26 (1948).

    Google Scholar 

  15. We thank F. Hoffmann-La Roche & Co., Basle (Switzerland), for the kind supply of the hemin used in these experiments.

  16. The leuco base can be prepared by the method ofGibbs et al. (H. D. Gibbs, B. Cohen, andR. K. Cannan, Public Health Reports40, 649 (1925), or by reducing a solution of dichlorophenolindophenol in 50% alcohol with the stoichiometric amount of ascorbic acid, followed by precipitation as a sodium salt by means of sodium chloride. It is purified by repeated dissolutions in alcohol, followed by precipitations with water. It must not contain traces of ascorbic acid, which will delay the reaction until it is oxidized.

    Article  CAS  Google Scholar 

  17. A. E. King, H. L. Roschen, andW. H. Irwin, Oil and Soap10, 105 (1933).

    Article  CAS  Google Scholar 

  18. S. Hartmann andJ. Glavind, Acta Physiol. Scand.16, suppl. 53, 32 (1948).

    Google Scholar 

  19. P. Gyorgy andH. Goldblatt, J. Exp. Med.75, 355 (1942).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. K. M. Endicott andR. D. Lillie, Amer. J. Pathol.20, 149 (1944).

    CAS  Google Scholar 

  21. H. Popper, P. Gyorgy, andH. Goldblatt, Arch. Pathol.37, 161 (1944).

    CAS  Google Scholar 

  22. R. D. Lillie, L. L. Ashburn, W. H. Sebrell, F. S. Daft, andJ. V. Lowry, Public Health Reports57, 502 (1942).

    Article  CAS  Google Scholar 

  23. K. M. Endicott, Arch. Pathol.37, 49 (1944).

    CAS  Google Scholar 

  24. T. Moore andY. L. Wang, Brit. J. Nutrition1, 53 (1947).

    Article  CAS  Google Scholar 

  25. K. E. Mason andA. F. Emmel, Yale J. Biol. Med.17, 189 (1944); Anat. Rec.92, 33 (1945).

    CAS  PubMed  PubMed Central  Google Scholar 

  26. K. E. Mason andI. R. Telford, Arch. Pathol.43, 363 (1947).

    CAS  Google Scholar 

  27. A. Wolf andA. M. Pappenheimer, J. Neuropathol. and Exp. Neurology4, 402 (1945).

    Article  CAS  Google Scholar 

  28. A. M. Pappenheimer andJ. Victor, Amer. J. Pathol.22, 395 (1946).

    Google Scholar 

  29. R. R. Bensley, Anat. Rec.98, 609 (1947).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Polytechnic Institute, Copenhagen

    J. Glavind, H. Granados, S. Hartmann & H. Dam

Authors
  1. J. Glavind
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  2. H. Granados
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  3. S. Hartmann
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  4. H. Dam
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Additional information

A preliminary report of this paper was presented at the 6th Scandinavian Physiological Congress, Acta Physiol. Scand.16, suppl. 53, 29 (1948).

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Glavind, J., Granados, H., Hartmann, S. et al. A histochemical method for the demonstration of fat peroxides. Experientia 5, 84–85 (1949). https://doi.org/10.1007/BF02153741

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  • DOI: https://doi.org/10.1007/BF02153741

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