Lipofuscin accumulation in extraocular muscle of rats deficient in vitamins E and A
The influences of vitamin E and A deficiencies on the formation of lipofuscin in two different muscle fiber types of the extraocular muscle were tested. Weanling female albino rats (Sprague-Dawley) were divided into three groups and fed purified diets that were adequate or deficient in vitamin E and A: +E, +A; −E, +A; and −E, −A. After 35 weeks on this diet, the animals were killed for analysis of extraocular muscle. When examined by fluorescence microscopy, the extraocular muscle of the (−E, +A) rats showed more lipofuscin-specific fluorescence than the (+E, +A) and (−E, −A) rats. Lipofuscin was then further analyzed by electron microscopy (EM), using morphometric analysis. By this high-resolution technique, the increased lipofuscin of the (−E, +A) extraocular muscle was seen to be confined mostly to the type I fibers. The type II fibers were quite insensitive to vitamin E deficiency: in type II fibers, the (−E, +A) and (−E, −A) muscle showed very small amounts of lipofuscin, and the (+E, +A) showed none at all. Vitamin A has an influence on vitamin E-deficiency and appears to be involved in the formation of lipofuscin in type I muscle fibers of the extraocular muscle.
KeywordsPublic Health Microscopy Electron Microscopy Muscle Fiber Fluorescence Microscopy
Unable to display preview. Download preview PDF.
- Bieri JG, Tolliver TJ, Robison WG Jr, Kuwabara T (1979) Lipofuscin in vitamin E deficiency and the possible role of retinol. Lipids 15:10–13Google Scholar
- Dowling JE, Gibbons IR (1961) The effect of vitamin A deficiency on the fine structure of the retina. In: Smelser GK (ed) The structure of the eye. Academic Press, New York, p 85Google Scholar
- Dowling JE, Wald G (1960) The biological function of vitamin A acids. Proc Natl Acad Sci USA 46:587Google Scholar
- Herrmann RK, Robison WG Jr, Bieri JG (1983) Vitamin E deficiency: lipofuscin accumulation in rat choroid and extraocular muscle. Invest Ophthalmol Vis Sci [ARVO Suppl] 24:181Google Scholar
- Katz ML, Robison WG Jr, Herrmann RK, Groome AB, Bieri JG (1984) Lipofuscin accumulation resulting from senescence and vitamin E deficiency: spectral properties and tissue distribution. Mech Age Dev 25:149–159Google Scholar
- McCay B, King MM (1980) Vitamin E: its role as a biological radical scavenger and its relationship to the microsomal mixed function oxidase system. In: Machlin LJ (ed) Vitamin E — a comprehensive treatise. Marcel Dekker, New York, pp 289–317Google Scholar
- Mendenhall W (1975) Introduction to probability and statistics. Wadsworth, Belmont, CaliforniaGoogle Scholar
- Nelson JS (1980) Pathology of vitamin E deficiency. In: Machlin LJ (ed) Vitamin E — a comprehensive treatise. Marcel Dekker, New York, pp 397–427Google Scholar
- Robison WG Jr, Katz ML, Bieri JG (1983) Dose-related influence of dietary vitamin A on lipofuscin accumulation in retinal pigment epithelium. Invest Ophthalmol Vis Sci [ARVO Suppl] 24: 181Google Scholar
- Scott ML (1969) Studies on vitamin E and related factors in nutrition and metabolism. In: Machlin LJ, Suttie JW (eds) The fat soluble vitamins. University of Wisconsin Press, Madison, pp 357–369Google Scholar
- Sohal RS (ed) (1981) Age pigments. Elsevier-North-Holland Biomedical Press, AmsterdamGoogle Scholar