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Experientia

, Volume 24, Issue 3, pp 222–223 | Cite as

Une note concernant la centrifugation fractionnée de foie de rat

  • P. Tulkens
  • R. Wattiaux
Specialia Chimica. Biochimica. Biophysica

Summary

Significant differences were observed in enzymic composition of L and P fractions between fasting and fed animals. The L fraction contains a smaller percentage and the P fraction a higher percentage of the enzymic activity of the homogenate when the rat is fed, which can be explained by the sedimentation in the L fraction of a high proportion of the glycogen accumulated in the liver of fed animals. The presence of this component changes the organization of the centrifugation pellet so that, during the decantation of the L fraction, subcellular structures sedimenting in this fraction are sucked off and recuperated in the next centrifugation pellet, i.e. in the microsomal P fraction.

References

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    C. de Duve, B. C. Pressman, R. Gianetto, R. Wattiaux etF. Appelmans, Biochem. J.60, 604 (1955).PubMedGoogle Scholar
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    O. H. Lowry, N. J. Rosenbrough, A. L. Farr etR. J. Randall, J. biol. Chem.193, 265 (1951).PubMedGoogle Scholar
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    S. Seifter, S. Dayton, B. Novic etE. Muntwyler, Archs Biochem. Biophys.25, 191 (1950).Google Scholar

Copyright information

© Birkhäuser Verlag 1968

Authors and Affiliations

  • P. Tulkens
    • 1
  • R. Wattiaux
    • 1
  1. 1.Laboratoire de chimie physiologique, Facultés universitaires Notre-Dame de la PaixNamurBelgique

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