Sexual Plant Reproduction

, Volume 9, Issue 5, pp 269–272 | Cite as

Arabinogalactan proteins at the cell surface ofBrassica sperm andLilium sperm and generative cells

Original Paper


Antibodies to arabinogalactan proteins were tested for binding to sperm cells ofBrassica campestris and to generative cells and sperm ofLilium longiflorum. Two monoclonal antibodies, JIM8 and JIM13, bound toBrassica sperm in pollen grains and pollen tubes and to isolated sperm. Sperm pairs retained within the vegetative cell inner plasma membrane fluoresced more brightly than single sperm, indicating that the vegetative cell inner plasma membrane that surrounds sperm pairs also contains arabinogalactan proteins. Isolated sperm pairs exhibited a uniform fluorescence while single sperm had patches of fluorescence. InLilium, isolated generative cells and single sperm cells bound antibodies in a patchy pattern. Antibodies to arabinogalactan proteins may be useful in describing the overall shape of sperm cells and for identifying sperm among other cell types.

Key words

Arabinogalactan proteins Brassica campestris Generative cell JIM8 JIM13 Lilium longiflorum Sperm (plant) 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brewbaker JL, Kwack BH (1963) The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot 50:859–865Google Scholar
  2. Dickinson DB (1965) Germination of lily pollen: respiration and tube growth. Science 150:1818–1819Google Scholar
  3. Faure J-E, Digonnet C, Dumas C (1994) An in vitro system for adhesion and fusion of maize gametes. Science 263: 1598–1600Google Scholar
  4. Harlow E, Lane D (1988) Antibodies, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 359–420Google Scholar
  5. Hough T, Singh MB, Smart IJ, Knox RB (1986) Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes. J Immunol Methods 92:103–108Google Scholar
  6. Knox JP, Linstead PJ, Peart J, Cooper C, Roberts K (1991) Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J 1:317–326Google Scholar
  7. Knox RB, Southworth D, Singh MB (1988) Sperm cell determinants and control of fertilisation in plants. In: Chapman GP, Ainsworth CC, Chatham CJ (eds) Eukaryote cell recognition: concepts and model systems. Cambridge University Press, Cambridge, pp 175–193Google Scholar
  8. McConchie CA, Jobson S, Knox RB (1985) Computer-assisted reconstruction of the male germ unit in pollen ofBrassica campestris. Protoplasma 127:57–63Google Scholar
  9. McConchie CA, Russell SD, Dumas C, Touhy M, Knox RB (1987) Quantitative cytology of the sperm cells ofBrassica campestris andB. oleracea. Planta 170:446–452Google Scholar
  10. Pennell RI (1992) Cell surface araginogalactan proteins, arabinogalactans and plant development. In: Callow JA, Green JR (eds) Perspectives in plant cell recognition. Cambridge University Press, Cambridge, pp 105–122Google Scholar
  11. Pennell RI, Roberts K (1990) Sexual development in the pea is presaged by altered expression of arabinogalactan protein. Nature 344:547–549Google Scholar
  12. Pennell RI, Geltz NR, Koren E, Russell SD (1987) Production and partial characterization of hybridoma antibodies elicited to the sperm ofPlumbago zeylanica. Bot Gaz 148:401–406Google Scholar
  13. Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K (1991) Developmental regulation of a plasma membrane arabinogalactan protein epitope in oilseed rape. Plant Cell 3:1317–1326Google Scholar
  14. Showalter AM (1993) Structure and function of plant cell wall proteins. Plant Cell 5: 9–23Google Scholar
  15. Southworth D (1992) Freeze fracture of male reproductive cells. Int Rev Cytol 140:187–204Google Scholar
  16. Southworth D, Jernstedt J (1995) Pollen exine development and microtubule rearrangement inVigna unguiculata (Fabaceae): a model for pollen wall patterning. Protoplasma 187:79–87Google Scholar
  17. Southworth D, Copley R, Kwiatkowski S (1992) Isolation of generative cells and sperm from pollen tubes ofLilium. In: Ottaviano E, Mulcahy DL, Sari-Gorla M, Mulcahy GB (eds) Angiosperm pollen and ovules. Springer, Berlin Heidelberg New York, pp 321–324Google Scholar
  18. Taylor PE, Kenrick J, Blomstedt CK, Knox RB (1991) Sperm cells of the pollen tubes ofBrassica: ultrastructure and isolation. Sex Plant Reprod 4:226–234Google Scholar
  19. Van Aelst AE, Van Went JL (1992) Ultrastructural immuno-localization of pectins and glycoproteins inArabidopsis thaliana pollen grains. Protoplasma 168:14–19Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  1. 1.Department of BiologySouthern Oregon State CollegeAshlandUSA

Personalised recommendations