Summary
Through a modified trypsin treatment of the surface of beef-erythrocytes, a substance was isolated with precipitation by a mixture of ethanol and ether, which did not contain significant amounts of hemoglobin and trypsin. A 6N HCl hydrolysate revealed with paper-chromatography 8 amino-acids in this substance. The surface of beef-erythrocyte membranes is covered with a protein- or peptide-layer.
Literatur
J. A. Morton undM. M. Pickles, Nature (Lond.)159, 779 (1947).
E. Ponder, Blood6, 350 (1951).
J. Tomcsik, Bull. schweiz. Akad. med. Wiss.16, 185 (1960).
E. Klenk undW. Stoffel, Hoppe-Seylers Z.303, 78 (1956).
G. F. Springer undM. J. Rapaport, Proc. Soc. exp. Biol. Med.96, 103 (1957).
E. M. W. Cook, D. H. Heard undE. V. F. Seamann, Nature (Lond.)188, 1011 (1960).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Scherrer-Gervai, M., Tomcsik, J. Isolierung und Charakterisierung einer Proteinsubstanz der Erythrocytenoberfläche. Experientia 18, 312–314 (1962). https://doi.org/10.1007/BF02151846
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02151846