Abstract
The distribution and morphology of intestinal endocrine cells was investigated in the mucosa of pelvic ileal reservoirs using immunocytochemical methods. Endoscopic biopsies were obtained from 15 patients after the construction of a modified J-pouch. The mucosa of the reservoir showed a variable degree of colonic metaplasia in all cases. No relevant quantitative variations of gut endocrine cells were detected, as revealed by immunostaining for the general marker, chromogranin, compared with normal ileal mucosa. Immunostaining for different peptide-containing cells resulted in normal number and morphology of serotonin, enteroglucagon, peptide tyrosine-tyrosine, and somatostatin-containing cells. Neurotensin cells were less numerous than in normal mucosa. The role played by gastrointestinal hormones in the adaptive response of the intestine to pouch construction is, presently, unclear. Further studies involving measurements of fasting and meal-stimulated levels of gut hormones in pouch patients might clarify this aspect.
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References
Parks AG, Nicholls RJ. Proctocolectomy without ileostomy for ulcerative colitis. Br Med J 1978;2:85.
Fonkalsrud EW. Update on clinical experience with different surgical techniques of the endorectal pull-through operation for colitis and polyposis. Surg Gynecol Obstet 1987;165:309–16.
Nicholls RJ. Restorative proctocolectomy with various types of reservoirs. World J Surg 1987;11:751–62.
Besterman HS, Adrian TE, Mallinson CN, et al. Gut hormones release after intestinal resection. Gut 1982;23:854–61.
Sagor GR, Gahatei MA, Al Mukhtar MY, Wright NA, Bloom SR. Evidence for a humoral mechanism after small intestinal resection: exclusion of gastrin but not enteroglucagon. Gastroenterology 1983;84:902–6.
Facer P, Bishop AE, Lloyd RV, Wilson BS, Hennessy RJ, Polak JM. Chromogranin: a newly recognized marker for endocrine cells of the gastrointestinal tract. Gastroenterology 1985;89:1366–73.
Stemberger LA. The unlabelled antibody peroxidase antiperoxidase (PAP) method. In: Immunocytochemistry. New York: John Wiley, 1979:104–69.
Buchan AM, Cohen Z, McLeod R, Greenberg GR. An immunocytochemical study of neurotensin, enteroglucagon and PYY containing cells after reconstructive surgery for ulcerative colitis in man. Gastroenterology 1985;88:1338.
Buchan AM, Griffiths CJ, Morris JF, Polak JM. Enteroglucagon cell hyperfunction in rat small intestine after gut resection. Gastroenterology 1985;88:8–12.
Lluis F, Thompson J. Neuroendocrine potential of the colon and rectum. Gastroenterology 1988;94:832–44.
Soria A, Beubler E. Neuropeptide Y (NPY) and peptide YY (PYY) inhibit prostaglandin E2 induced intestinal fluid and electrolyte secretion in the rat jejunum in vivo. Eur J Pharmacol 1985;1119:47–52.
Adrian TE, Savage AP, Fuessl HS, Wolfe K, Besterman HS, Bloom SR. Release of peptide Y-Y after resection of small bowel, colon or pancreas in man. Surgery 1987;101:715–9.
Olsen PS, Pederson JH, Paulsen SS, Yamashita Y, Kirkegaard P. Neurotensin like immunoreactivity after intestinal resection in the rat. Gut 1987;28:1107–11.
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Pietroletti, R., Slors, J.F.M., Carlei, F. et al. Immunocytochemical study of endocrine cells in pelvic ileal reservoirs. Dis Colon Rectum 33, 703–706 (1990). https://doi.org/10.1007/BF02150749
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DOI: https://doi.org/10.1007/BF02150749