Summary
In the presence of aceto acetic acid the evaluation of pyruvic acid with 2,4-dinitrophenylhydrazin is disturbed because 6–8% of aceto acetic acid are determined as coloured hydrazon with the usual method. The elimination of aceto acetic acid can be obtained by putting the filtrate after deproteinization with trichloracetic acid into boiling water during 5 minutes. Less than 10% of pyruvic acid are destroyed by this procedure.
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Markees, S. Die Ausschaltung des Azetessigsäurefehlers bei der Brenztraubensäurebestimmung. Experientia 7, 314–315 (1951). https://doi.org/10.1007/BF02150563
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DOI: https://doi.org/10.1007/BF02150563