Summary
A method is described by means of which radiocalcium and radiophosphorus can be used simultaneously in biological tracer work. The chemical separation is eliminated when the different beta energies of these two isotopes are used. An aluminium absorber of 0.5 mm stops all of the45Ca-β-rays, but reduces the activity of the32P by a factor of about 2.7. A sample containing both isotopes is first measured without absorber, giving the activity due to both isotopes; the activity measured with absorber multiplied by 2.7, will give the32P contribution; the45Ca contribution is then calculated from the difference.
Literatur
W. D. Armstrong etC. P. Barnum, J. Biol. Chem.172, 199 (1948).
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Govaerts, J. Méthode de dosage du45Ca et du32P au cours de l'emploi simultané des deux radioisotopes. Experientia 7, 312–314 (1951). https://doi.org/10.1007/BF02150562
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DOI: https://doi.org/10.1007/BF02150562