Cellular and Molecular Neurobiology

, Volume 16, Issue 4, pp 517–528 | Cite as

Myelin-stimulated macrophages release neurotrophic factors for adult dorsal root ganglion neurons in culture

  • Naoshi Hikawa
  • Toshifumi Takenaka


  1. 1.

    Our previous study demonstrated that cultured macrophages release neurotrophic factors spontaneously. In a histological study of Wallerian degeneration, macrophages phagocytosed myelin debris and expressed activated markers.

  2. 2.

    To investigate the role of myelin-stimulated macrophages on neurite regeneration, we prepared conditioned media from cultured mouse peritoneal macrophages which had phagocytosed a myelin fraction. This conditioned media enhanced both neurone survival and neurite regeneration of adult dorsal root ganglia (DRG) neurons compare to conditioned media from macrophage cultures without myelin.

  3. 3.

    The production of the neurotrophic supernatant was dose-dependent on myelin fraction and specific for myelin because supernatants from macrophages incubated with LPS (lipopolysaccharide), MDP (N-acetylmuramyl-L-alanyl-D-isoglutamine) or latex beads were not neurotrophic.

  4. 4.

    The neurotrophic factors from myelin-stimulated macrophages were different from spontaneously released macrophage factors as they differed in heat-sensitivity.

  5. 5.

    These results suggest that myelin-stimulated macrophages contribute to axon regeneration after Wallerian degeneration.


Key Words

neurite regeneration Wallerian degeneration lipopolysaccharide N-acetylmuramyl-L-analyl-D-isoglutamine sensory neuron phagocytosis 


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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Naoshi Hikawa
    • 1
  • Toshifumi Takenaka
    • 1
  1. 1.Department of Physiology, School of MedicineYokohama City UniversityYokohamaJapan

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