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Expression of platelet-derived growth factor (PDGF) receptor α-subunit in mouse brain: Comparison ofPatch mutants and normal littermates

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Summary

  1. 1.

    Platelet-derived growth factor (PDGF) plays an important role not only in mesenchyme-derived tissues, but also in the mammalian central nervous system. ThePatch mutant (Ph/+) lacks one copy of the PDGFR-α gene. However, it is not clear whether there are differences in expression of PDGF receptor α-subunit (PDGFR-α) in brain tissue of thePatch heterozygous (Ph/+) mutants compared to wild-type C57B1 (+/+) mice.

  2. 2.

    The level of PDGRF-α mRNA expression is slightly lower inPatch mutant than in normal littermate.

  3. 3.

    Protein and total RNA isolated from mouse brain tissue and primary type 1 astrocyte cultures were studied with Western and Northern blotting techniques. There was no measurable difference in PDGFR-α protein expression between thePatch and wild-type mouse nervous system. Adjustment of transcriptional efficiency and messenger stability may contribute to this phenomenon, whose biological significance remains unclear.

  4. 4.

    Further, the expression of PDGRF-α protein and message in mouse brain tissues is developmentally regulated. Its level remains high during the embryonic period and declines below measurable levels in adult.

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Authors and Affiliations

  1. Department of Anatomy, University of Mississippi Medical Center, 2500 North State Street, 39216-4500, Jackson, Mississippi

    Frank X. Zhang & James B. Hutchins

  2. Department of Neurology, University of Mississippi Medical Center, 2500 North State Street, 39216-4500, Jackson, Mississippi

    James B. Hutchins

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  1. Frank X. Zhang
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  2. James B. Hutchins
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Zhang, F.X., Hutchins, J.B. Expression of platelet-derived growth factor (PDGF) receptor α-subunit in mouse brain: Comparison ofPatch mutants and normal littermates. Cell Mol Neurobiol 16, 479–487 (1996). https://doi.org/10.1007/BF02150228

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  • DOI: https://doi.org/10.1007/BF02150228

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