Summary
Pea plants (Pisum sativum L., var. Lincoln) were grown in nutrient cultures at 4 levels of iron, 0.60 ppm (low), 0.96 ppm (low), 3.0 ppm (normal) and 30 ppm (excess) for 45 days.
Leaf extracts were assayed for chlorophyll, proteins, catalase and peroxidase activities. Catalase and chlorophyll were closely related to iron supply. An inverse relationship was observed between peroxidase and catalase activities. Peroxidase was increased both at dificiency and excess iron levels, but was depressed at normal iron supply. The peroxidase/catalase ratio varied with iron supply and showed a minimum value of about 39 at 15 and 30 days growth, at adequate iron supplies.
Measurement of catalase activity and the use of peroxidase/catalase ratios appear to be helpful in identifying iron deficiencies in peas.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agarwala, S. C., Sharma, C. P. and Kumar, A., Interrelationship of iron and manganese supply in growth, chlorophyll and iron porphyrin enzymes in barley plants. Plant Physiol.39, 603–9 (1964).
Agarwala, S. C., Sharma, C. P. and Farooq, S., Effect of iron supply on growth, chlorophyll, tissue iron and activity of certain enzymes in maize and radish Plant Physiol.40, 493–9 (1965).
Arnon, D. I., Copper enzymes in isolated chloroplasts. Plant Physiol.24, 1–45 (1949).
Bar-Akiva, A., Lavon, R. and Sagiv, J., Ascorbic acid oxidase activity as a measure of the copper nutrition requirement of citrus trees. Agrochimica14, 47–54 (1969).
Bar-Akiva, A., The use of activity of metallo-enzyme systems for the appraisal of nutritional requirements of citrus tress. Proc. First Int. Citrus Symp.3, 1551–7 (1969).
Bar-Akiva, A., Functional aspects of mineral nutrients in use for the evaluation of plant nutrient requirement.In Recent Advances in Plant Nutrition (Samish, R. M., ed., Gordon and Breach Science Publishers, New York)I, 115–142 (1971).
Birecka, H. and Galston, A. W., Peroxidase ontogeny in a dwarf pea stem as affected by gibberellin and decapitation. J. Exp. Bot.21, 735–45 (1970).
Brown, J. C. and Hendricks, S. B., Enzymic activities as indicators of Cu and Fe deficiencies in plants. Plant Physiol.27, 651–60 (1952).
Comité Inter-Institutos para el estudio de técnicas analíticas de diagnóstico foliar. Méthodes de référence pour la détermination des éléments minéraux dans les végétaux. Détermination des éléments Ca, Mg, Fe, Mn, Zn et Cu par absorption atomique. Oléagineux28, 87–92 (1973).
Dekock, P. C., Commisiong, K., Farmer, V. C. and Inkson, R. H. E., Interrelationships of catalase, peroxidase, hematin and chlorophyll. Plant Physiol.35, 599–604 (1960).
Del Rio, L. A., Gomez, M., Leal, A., and Lopez Gorge, J., A more sensitive modification of the catalase assay with the Clark oxygen electrode. Application to the kinetic study of the pea leaf enzyme. Anal. Biochem.80, 409–15 (1977).
Gardiner, M. G. and Cleland, R., Peroxidase changes during the cessation of elongation inPisum sativum stems. Pytochemistry13, 1095–8 (1974).
Goodall, D. V. and Gregory, F. G., Chemical composition of plants as an index of their nutritional status. Tech. Commun. Commonw. Bur. Hort. Plant. Crops No.17 (1947).
Hewitt, E. J., Sand and Water Culture Methods Used in the Study of Plant Nutrition (Edited by the Commonw. Bur. Hort. Plant. Crops, C.A.B., Farham Royal. Bucks., England) 2nd edition, p. 431 (1966).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–75 (1951).
Marsh, H. V., Evans, H. J. and Matrone, G., Investigations of the role of iron in chlorophyll metabolism. I. Effect of iron deficiency on chlorophyll and heme content and on the activity of certain enzymes in leaves. Plant Physiol.38, 632–8 (1963).
O'Sullivan, M., Aldolase activity in plants as an indicator of zinc deficiency. J. Sci. Food Agric.21, 607–9 (1970).
O'Sullivan, M., Enzyme studies in diagnosing nutrient deficiencies. Potassium Institute Ltd., Colloquium Proc. No.2, 1–6 (1972).
Potty, V. H., Determination of proteins in the presence of phenols and pectins. Anal. Biochem.29, 535–9 (1969).
Price, C. A., Iron compounds and plant nutrition. Annu. Rev. Plant Physiol.19, 239–48 (1968).
Ulmer, R. L., Haskins, F. A. and Akeson, W. R., Influence of iron supply on peroxidase activity and peroxidase isozymes in corn (Zea mays). Crop Science11, 816–8 (1971).
Weinstein, L. H. and Robbins, W. R., The effect of different iron and manganese nutrient levels on the catalase and cytochrome oxidase activities of green and albino sunflower leaf tissues. Plant Physiol.30, 27–32 (1955).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Del Rio, L.A., Gomez, M., Yañez, J. et al. Iron deficiency in pea plants effect on catalase, peroxidase, chlorophyll and proteins of leaves. Plant Soil 49, 343–353 (1978). https://doi.org/10.1007/BF02149743
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02149743