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A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate

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Abstract

A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenie substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

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Authors and Affiliations

  1. Departments of Bacteriology and Immunology and Surgery, Western Infirmary, G11 6NT, Glasgow, Scotland

    B. I. Piotrowicz, I. Watt, S. Edlin & A. C. McCartney

Authors
  1. B. I. Piotrowicz
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  2. I. Watt
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  3. S. Edlin
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  4. A. C. McCartney
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Piotrowicz, B.I., Watt, I., Edlin, S. et al. A micromethod for endotoxin assay in human plasma using limulus amoebocyte lysate and a chromogenic substrate. Eur. J, Clin. Microbiol. 4, 52–54 (1985). https://doi.org/10.1007/BF02148661

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  • DOI: https://doi.org/10.1007/BF02148661

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