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Quantitative studies on the influence of the temperature applied in freeze-substitution on P, N, and dry mass losses in fixed tissues

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Résumé

Les auteurs ont constaté que la température a une influence sur la diminution du contenu en azote et en phosphore et sur celle de la masse déséchée après l'usage de méthanol au cours de la congelation-dissolution (freeze-substitution) des tissus. Ces diminutions sont moins marquées à la temperature de − 79°C. Après l'usage d'acétone, les diminutions atteignent leur minimum.

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Literatur

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Authors and Affiliations

  1. Department of Histology, School of Medicine, Warsaw, Poland

    K. Ostrowski, J. Komender, H. Košcianek & K. Kwarecki

Authors
  1. K. Ostrowski
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  2. J. Komender
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  3. H. Košcianek
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  4. K. Kwarecki
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Ostrowski, K., Komender, J., Košcianek, H. et al. Quantitative studies on the influence of the temperature applied in freeze-substitution on P, N, and dry mass losses in fixed tissues. Experientia 18, 227–228 (1962). https://doi.org/10.1007/BF02148314

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  • DOI: https://doi.org/10.1007/BF02148314

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