Summary
The cells obtained from anaerobic cultures ofA. aerogenes contain both a hydrogenase and a fumaric reductase, but lack one or several electron transporters necessary for the coupling of the two enzymes. It is possible to measure the fumaric reductase activity of the extracts in the presence of hydrogen, hydrogenase and benzyl viologen, using the Warburg manometric technique. In anaerobic cultures the reductase is an inducible enzyme. In aerobic cultures its formation is strongly repressed by atmospheric oxygen.
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Pichinoty, F., Coudert, G. Action répressive de l'oxygène sur la biosynthèse de la fumarique-réductase d'Aerobacter aerogenes. Experientia 18, 257–259 (1962). https://doi.org/10.1007/BF02148218
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DOI: https://doi.org/10.1007/BF02148218