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Experientia

, Volume 24, Issue 11, pp 1183–1184 | Cite as

Fluorescence assay of tyrosine hydroxylase activity in tissue homogenate

  • T. Nagatsu
  • T. Yamamoto
Specialia Pro Experimentis

Keywords

Tyrosine Tyrosine Hydroxylase Tissue Homogenate Hydroxylase Activity Fluorescence Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Es wird eine Fluoreszenzmethode zur Bestimmung der Tyrosin-Hydroxylase-Aktivität von Homogenat beschrieben, die auf der Spektrofluorometrie der DOPA-Bildung beruht. Die Tyrosin-Hydroxylase-Aktivität von Homogenat der Nebenniere und des Gehirns (Nucleus caudatus) wurde mit dieser Methode gemessen.

Literatur

  1. 1.
    T. Nagatsu, M. Levitt andS. Udenfriend, J. biol. Chem.239, 2910 (1964).PubMedGoogle Scholar
  2. 2.
    T. Nagatsu, M. Levitt andS. Udenfriend, Anal. Biochem.9, 122 (1964).Google Scholar
  3. 4.
    The optimum temperature for tyrosine hydroxylase activity in this incubation mixture was found to be at 30°C.S. Ayukawa, T. Takeuchi, T. Sezaki, H. Hara, H. Umezawa andT. Nagatsu, J. Antibiotics21, 350 (1968).Google Scholar
  4. 5.
    S. Kaufman, Proc. Natn Acad. Sci. (USA)50, 1085 (1963).Google Scholar
  5. 6.
    R. Crout, inStandard Methods of Clinical Chemistry (Ed.D. Seligson, Academic Press, New York 1961), vol. 3, p. 62.Google Scholar
  6. 7.
    U. S. von Euler andI. Floding, Acta Physiol. Scand.33, Suppl.118, 45 (1955).PubMedGoogle Scholar
  7. 8.
    S. Udenfriend, inFluorescence Assay in Biology and Medicine (Academic Press, New York 1962), p. 136.Google Scholar

Copyright information

© Birkhäuser Verlag 1968

Authors and Affiliations

  • T. Nagatsu
    • 1
  • T. Yamamoto
    • 1
  1. 1.Department of Biochemistry, School of DentistryAichi-Gakuin UniversityNagoyaJapan

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