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Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system

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Abstract

Expression of the c-sis oncogene, the gene encoding the B chain of platelet-derived growth factor (PDGF), may be related to initiation and/or progression of glial cell tumorigenesis by PDGF-mediated autocrine growth stimulation. As the mechanism for activation of expression of the c-sis gene in gliomas is not known, we searched for possible structural alterations of c-sis DNA in these tumors. Genomic Southern blots of DNA from 7 different cultured human glioblastoma cell lines and 15 different solid human brain tumors revealed no significant change in either the gross structure or the copy number of the c-sis gene in tumor cells vs. control cells. Activation of glioma c-sis gene expression is therefore not the result of a gross rearrangement or amplification of the c-sis gene. Expression of c-sis mRNA was detected in all of 12 different solid human brain tumors, 11 of which were of glial cell origin. However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.

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Authors and Affiliations

  1. Department of Biochemistry, Case Western Reserve University, 44106, Cleveland, OH, USA

    Richard D. Press, Anita Misra, David Samols & David A. Goldthwait

  2. Department of Neurosurgery, University Hosptials, 44106, Cleveland, OH, USA

    Timothy B. Mapstone

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  1. Richard D. Press
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  2. Anita Misra
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  3. David Samols
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  4. David A. Goldthwait
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  5. Timothy B. Mapstone
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Press, R.D., Misra, A., Samols, D. et al. Major structural alterations of the c-sis gene are not observed in a series of tumors of the human central nervous system. J Neuro-Oncol 7, 345–356 (1989). https://doi.org/10.1007/BF02147092

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