Zusammenfassung
Durch wiederholtes Waschen mit hypotonen Lösungen gelingt es, mit dem Hämoglobin auch die Carboanhydrase vollständig aus Erythrocytenstromata zu entfernen.
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Literatur
T. Enns, Science155, 44 (1967).
G. De Stevens,Diuretics (Academic Press, New York 1963).
Based on method ofE. T. Dunham andI. M. Glynn,J. Physiol. 156, 274 (1961), the hemolyzing and washing solution contained 10−4 M cysteine, 10−3 M EDTa (ethylenediamine tetraacetate) and 10−4 M Tris-Ac (Tris(hydroxymethyl) aminomethaneacetate) at a final pH of 7.4.
The cells were treated with 1:5, 1:6, 1:7 and 1:8 dilutions (1 vol. NaCl mix plus 4 vol. H2O, etc.) of the following: 4 × 10−4 M cysteine and 2 × 10−3 M EDTA in 9 vol. 0.88% NaCl plus 1 vol. 0.15M Tris-Ac, pH 7.4.R. Blomfield, C. Long, E. J. Sargent andA. B. Sidle, Biochem. J.101, 44P (1966).
D. V. Tappan, M. J. Jacey andH. M. Boyden, Ann. N.Y. Acad. Sci.121, 589 (1964).
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Tappan, D.V. Carbonic anhydrase activity of erythrocyte ghosts. Experientia 24, 127 (1968). https://doi.org/10.1007/BF02146936
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DOI: https://doi.org/10.1007/BF02146936