Experientia

, Volume 24, Issue 2, pp 127–127 | Cite as

Carbonic anhydrase activity of erythrocyte ghosts

  • D. V. Tappan
Specialia Chimica. Biochimica. Biophysica

Keywords

Ghost Carbonic Anhydrase Carbonic Anhydrase Activity Erythrocyte Ghost 

Zusammenfassung

Durch wiederholtes Waschen mit hypotonen Lösungen gelingt es, mit dem Hämoglobin auch die Carboanhydrase vollständig aus Erythrocytenstromata zu entfernen.

Literatur

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    T. Enns, Science155, 44 (1967).PubMedGoogle Scholar
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    G. De Stevens,Diuretics (Academic Press, New York 1963).Google Scholar
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    Based on method ofE. T. Dunham andI. M. Glynn,J. Physiol. 156, 274 (1961), the hemolyzing and washing solution contained 10−4 M cysteine, 10−3 M EDTa (ethylenediamine tetraacetate) and 10−4 M Tris-Ac (Tris(hydroxymethyl) aminomethaneacetate) at a final pH of 7.4.PubMedGoogle Scholar
  4. 4.
    The cells were treated with 1:5, 1:6, 1:7 and 1:8 dilutions (1 vol. NaCl mix plus 4 vol. H2O, etc.) of the following: 4 × 10−4 M cysteine and 2 × 10−3 M EDTA in 9 vol. 0.88% NaCl plus 1 vol. 0.15M Tris-Ac, pH 7.4.R. Blomfield, C. Long, E. J. Sargent andA. B. Sidle, Biochem. J.101, 44P (1966).Google Scholar
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    D. V. Tappan, M. J. Jacey andH. M. Boyden, Ann. N.Y. Acad. Sci.121, 589 (1964).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1968

Authors and Affiliations

  • D. V. Tappan
    • 1
  1. 1.Submarine Medical CenterGrotonUSA

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