, Volume 24, Issue 2, pp 127–127 | Cite as

Carbonic anhydrase activity of erythrocyte ghosts

  • D. V. Tappan
Specialia Chimica. Biochimica. Biophysica


Ghost Carbonic Anhydrase Carbonic Anhydrase Activity Erythrocyte Ghost 
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Durch wiederholtes Waschen mit hypotonen Lösungen gelingt es, mit dem Hämoglobin auch die Carboanhydrase vollständig aus Erythrocytenstromata zu entfernen.


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    Based on method ofE. T. Dunham andI. M. Glynn,J. Physiol. 156, 274 (1961), the hemolyzing and washing solution contained 10−4 M cysteine, 10−3 M EDTa (ethylenediamine tetraacetate) and 10−4 M Tris-Ac (Tris(hydroxymethyl) aminomethaneacetate) at a final pH of 7.4.PubMedGoogle Scholar
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    The cells were treated with 1:5, 1:6, 1:7 and 1:8 dilutions (1 vol. NaCl mix plus 4 vol. H2O, etc.) of the following: 4 × 10−4 M cysteine and 2 × 10−3 M EDTA in 9 vol. 0.88% NaCl plus 1 vol. 0.15M Tris-Ac, pH 7.4.R. Blomfield, C. Long, E. J. Sargent andA. B. Sidle, Biochem. J.101, 44P (1966).Google Scholar
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Copyright information

© Birkhäuser Verlag 1968

Authors and Affiliations

  • D. V. Tappan
    • 1
  1. 1.Submarine Medical CenterGrotonUSA

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