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An improved procedure for purification of alkaline phosphatase from rat kidney

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  • Chimica. Biochimica. Biophysica
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Riassunto

Viene descitto un nuovo metodo per la purificazione della fosfatasi alcalina da rene di ratto. La metodica comprende 4 tappe: (1) isolamento della frazione microsomiale; (2) solubilizzazione dell'enzima dalle strutture microsomiali per mezzo del desossicolato e della lipasi; (3) frazionamento cromatografico su DEAE-Sephadex A-25 ed eluzione con gradiente di concentrazione; (4) ricromatografia delle frazioni attive sullo stesso scambiatore anionico. All'elettroforesi su gel d'amido l'enzima ottenuto si comporta come una proteina omogenea.

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Literatur

  1. F. Melani, G. Ramponi, A. Guerritore andV. Baccari, Nature201, 710 (1964).

    Article  CAS  Google Scholar 

  2. F. Melani, G. Ramponi, M. Farnararo, E. Cocucci andA. Guerritore, Biochim. biophys. Acta138, 411 (1967).

    Article  CAS  Google Scholar 

  3. F. Melani, M. Farnararo andG. Sgaragli, Archs Biochem. Biophys., in press.

  4. R. K. Morton, Biochem. J.57, 595 (1954).

    Article  CAS  Google Scholar 

  5. P. Portmann, Hoppe-Seyler's Z. physiol. Chem.309, 87 (1957).

    Article  CAS  Google Scholar 

  6. E. F. Alvarez andM. Lora-Tamayo, Biochem. J.69, 312 (1958).

    Article  CAS  Google Scholar 

  7. J. C. Mathies, J. biol. Chem.233, 1121 (1958).

    Article  CAS  Google Scholar 

  8. O. Schales andK. Arai, Archs Biochem. Biophys.83, 152 (1959).

    Article  CAS  Google Scholar 

  9. A. Garen andC. Levinthal, Biochim. biophys. Acta38, 470 (1960).

    Article  CAS  Google Scholar 

  10. Z. Ahmed andJ. King, Biochim. biophys. Acta40, 320 (1960).

    Article  CAS  Google Scholar 

  11. M. H. Malamy andB. L. Horecker, Biochemistry3, 1893 (1964).

    Article  CAS  Google Scholar 

  12. D. Dabich andO. W. Neuhaus, J. biol. Chem.241, 415 (1966).

    Article  CAS  Google Scholar 

  13. D. W. Moss, R. H. Eaton, J. K. Smith andL. G. Whithy, Biochem. J.102, 53 (1967).

    Article  CAS  Google Scholar 

  14. F. Melani andA. Guerritore, Experientia20, 464 (1964).

    Article  CAS  Google Scholar 

  15. O. Smithies, Biochem. J.71, 585 (1959).

    Article  CAS  Google Scholar 

  16. G. Beisenhertz, H. J. Boltze, T. Bücher, R. Czok, R. K. Garbade, E. Meyer-Arendt andG. Pfleiderer, Z. Naturf.8b, 555 (1953).

    Article  Google Scholar 

  17. O. Warburg andW. Christian, Biochem. Z.310, 384 (1941).

    Google Scholar 

  18. R. K. Morton, Biochem. J.55, 786 (1953).

    Article  CAS  Google Scholar 

  19. M. Martland andR. Robinson, Biochem. J.23, 237 (1929).

    Article  CAS  Google Scholar 

  20. H. Albers andE. Albers, Hoppe-Seyler's Z. physiol. Chem.232, 47, 165, 189 (1935).

    Article  Google Scholar 

  21. M. A. M. Abul-Fadl andE. J. King, Biochem. J.44, 435 (1949).

    Article  CAS  Google Scholar 

  22. R. K. Morton, Nature172, 65 (1953).

    Article  CAS  Google Scholar 

  23. R. K. Morton, Biochem. J.60, 573 (1955).

    Article  CAS  Google Scholar 

  24. F. Melani andM. Farnararo, Abstract Soc. Ital. Biochim., Siena (1966).

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Authors and Affiliations

  1. Institute of Biochemistry, University of Firenze, Italy

    F. Melani, M. Farnararo & G. Sgaragli

Authors
  1. F. Melani
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  2. M. Farnararo
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  3. G. Sgaragli
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Additional information

This work was supported by a grant from the Italian Consiglio Nazionale delle Ricerche, Impresa Enzimologia.

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Melani, F., Farnararo, M. & Sgaragli, G. An improved procedure for purification of alkaline phosphatase from rat kidney. Experientia 24, 114–115 (1968). https://doi.org/10.1007/BF02146928

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  • DOI: https://doi.org/10.1007/BF02146928

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