Zusammenfassung
Es wird eine Methode zur quantitativen Analyse von Ribosederivaten beschrieben, welche etwa fünftausendmal empfindlicher ist als die spektrophotometrischen Standardverfahren.
Literatur
K. Randerath, Analyt. Biochem.21, 480 (1967).
J. X. Khym andW. E. Cohn, J. Am. chem. Soc.82, 6380 (1960).
Abbreviations: AR, adenosine; CR, cytidine; UR, uridine; GR, guanosine; AR', CR', UR', GR', the corresponding nucleoside trialcohol derivatives obtained by periodate oxidation and borohydride reduction.
C. F. Gordon andA. L. Wolfe, Analyt. Chem.32, 574 (1960).
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This work was supported by grants-in-aid from the U.S. Atomic Energy Commission (No. AT(30-1)2643), the U.S. Public Health Service (No. CA 05018), and the American Cancer Society (No. E-51-J). This is publication No. 1325 of the Cancer Commission of Harvard University.
The first paper of this series3 described a procedure for detecting subnanomolar quantities of mono- and oligonucleotides on PEI-cellulose thin layers.
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Randerath, K., Randerath, E. Analysis of nucleic acid derivatives at the subnanomolar level. II. Quantitative analysis of ribose derivatives by tritium labeling. Experientia 24, 1192–1193 (1968). https://doi.org/10.1007/BF02146613
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DOI: https://doi.org/10.1007/BF02146613