Antonie van Leeuwenhoek

, Volume 6, Issue 1, pp 251–262 | Cite as

The formation of acetic acid from carbon dioxide and hydrogen by anaerobic spore-forming bacteria

  • K. T. Wieringa


Further experiments on an anaerobic bacillus synthesising acetic acid from CO2 and H2 are described. The organism in question was classified asClostridium aceticum n.sp. Acetic acid is also formed from sugar.

It was shown, that at any moment the number of H2 molecules used for synthesis is proportional to the number of molecules present. Continuous provision with H2 and CO2 influences the rate of the process favourably. In such conditions a culture may absorb as much as 4.5 1 of H2 a day at 30‡ C.

The pH range ofCl. aceticum is between 7.5 and 10.5, the optimum being between 8 and 9.

A growth promoting substance is present in alkaline mud extract. This substance can be concentrated by means of absorption or precipitation. Its nature is still unknown.


Hydrogen Acetic Sugar Dioxide Acetic Acid 
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Copyright information

© N. V. Swets en Zeitlinger Boekhandel en Eutgeversmaatschappij 1939

Authors and Affiliations

  • K. T. Wieringa
    • 1
  1. 1.Microbiological LaboratoryAgricultural UniversityWageningen

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