Summary
SixCytospora isolates were cultured on laboratory media over an initial pH range of 8.0 to 4.0.
The optimum pH was found to be approximately 4.2. However, since the autoclaving process shifts pH values in the acid direction, adjustment of media to pH 4.8 is necessary prior to sterilization.
Growth processes of the isolates were demonstrated to have a similar acidifying effect on the medium, but this effect was much reduced at 5.5 and lower pH levels.
In summarizing the results of the series of investigations dealing with optimum conditions of laboratory environment; the authors recommend culturingCytopora fungi at 28° C, on modified Lilly and Barnett media containing maltose and potassium nitrate as carbon and nitrogen sources, at a pH level of 4.2.
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Literature Cited
Helton, A. W. &D. E. Konicek, 1962. An optimum environment for the culturing ofCytospora isolates from stone fruits. I. Temperature. Mycopathol. et Mycol. Appl.,16, 19–26.
Helton, A. W. &D. E. Konicek, 1962. An optimum environment for the culturing ofCytospora isolates from stone fruits. III. Nitrogen sources. Mycopathol. et Mycol. Appl.,16, 125–132.
Helton, A. W. &D. E. Konicek, 1961. Effects of selectedCytospora isolates from stone fruits on certain stone fruit varieties. Phytopathology51: 152–157.
Helton, A. W. &J. A. Moisey, 1955.Cytospora damage in Idaho prune orchards. Plant Disease Reptr.39: 931–943.
Konicek, D. E. &A. W. Helton, 1962. An optimum environment for the culturing ofCytospora isolates from stone fruits. II. Carbon sources. Mycopathol. et Mycol. Appl.,16, 27–34.
Lilly, V. G. &H. L. Barnett, 1951. Physiology of the fungi. McGraw-Hill Co., Inc. New York. 464 p.
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Approved by the Director of the Idaho Agricultural Experiment Station as Research Paper No. 520.
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Konicek, D.E., Helton, A.W. An optimum environment for the culturing of Cytospora isolates from stone fruits. IV. Hydrogen-ion concentration. Mycopathologia et Mycologia Applicata 16, 243–248 (1962). https://doi.org/10.1007/BF02145736
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DOI: https://doi.org/10.1007/BF02145736