Summary
An inverse, almost linear, relationship was found between the temperature of frozen mycobacterial paste and the hydrostatic pressure necessary for disintegration of mycobacterial cells in pressure chambers. The optimal reproducible values of temperature pressure for the quantitative isolation of mycobacterial nucleoproteins were estimated in the range of −30 to −40°C per 3000–5000 kg/cm2.
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Staflová, S., Trnka, L. & Nejedlý, J. Einfluss der Unterkühlung auf die Desintegrierung der Tuberkelbakterien in Druckkammern. Experientia 21, 624 (1965). https://doi.org/10.1007/BF02144041
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DOI: https://doi.org/10.1007/BF02144041