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Enzyme activities in the rabbit cornea during immunogenic keratitis

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Abstract

In rabbits inflammation of the cornea was induced by intrastromal injection of horse serum. Between 2 and 4 weeks after injection, infiltration of the cornea with leukocytes and neovascularization could be observed. During this period, the rabbits were killed and their corneas analyzed for protein, alkaline phosphatase, acid phosphatase,N-acetyl-β-D-glucosaminidase, and lactate dehydrogenase. The enzyme activities in the inflamed corneal stroma reflect the high lysosomal activity, which probably originates from the leukocytes. The enzyme activities in the epithelium indicate that the tissue is abnormal and undergoing repair processes.

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References

  • Bolkova A (1977) Biochemical study on some acid hydrolases in the normal rabbit eye. Graefe's Arch Clin Exp Ophthalmol 203:67–71

    Article  Google Scholar 

  • Bolkova A, Obenberger J (1976) Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury. Graefe's Arch Clin Exp Ophthalmol 200:251–255

    Article  Google Scholar 

  • Breebaart AC (1960) Experimental corneal anaphylaxis. Thesis, University of Amsterdam, The Netherlands

  • Cejkova J, Bolkova A (1977) A study on alkaline phosphatase in cornea of various animals with special regards to keratocytes. Graefe's Arch Clin Exp Ophthalmol 204:209–214

    Article  Google Scholar 

  • Chayakul V, Reim M (1982) Enzymatic activity of β-N-acetylglucosaminidase in the alkali-burned rabbit cornea. Graefe's Arch Clin Exp Opthalmol 218:149–152

    Google Scholar 

  • Kaplunovich PS, Eremina II, Kolina MB, Skryabina GN (1973a) Alkaline phosphatase and inorganic phosphorus content in the cornea following chemical burns. Vestn Ofthalmol 6:40–42

    Google Scholar 

  • Lowry OR, Rosenborough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  • Morawiecki J (1956) Präzipitationserscheinungen in der lebenden Hornhaut bei Antigenantikörperreaktionen. Ophthalmilogica 132:236–242

    Google Scholar 

  • Raekallio J (1970) Enzyme histochemistry of wound healing. Prog. Histochem Cytochem 1: 51–152

    Google Scholar 

  • Smolin G, Okumoto M, Hall J, Friedlaender M (1979) The effect of indoxole on the corneal immunologic response. Ann Ophthalmol 11:227–232

    PubMed  Google Scholar 

  • Van Haeringen NJ; Oosterhuis JA, Van Delft JL, Barthen ER (1983) Inhibitors of prostaglandin synthesis and the cornea. Doc Ophthalmol 56:23–25

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Biochemical Laboratory, The Netherlands Ophthalmic Research Institute, P.O. Box 12141, 1100 AC, Amsterdam, The Netherlands

    N. J. van Haeringen

  2. Institute of Experimental Medicine, Czechoslovac Academy of Sciences, Praha, Czechoslovakia

    A. Bolkova

  3. Department of Ophthalmology, Leiden Medical Center, Leiden, The Netherlands

    J. A. Oosterhuis & J. L. van Delft

Authors
  1. N. J. van Haeringen
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  2. A. Bolkova
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  3. J. A. Oosterhuis
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  4. J. L. van Delft
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van Haeringen, N.J., Bolkova, A., Oosterhuis, J.A. et al. Enzyme activities in the rabbit cornea during immunogenic keratitis. Graefe's Arch Clin Exp Ophthalmol 224, 288–290 (1986). https://doi.org/10.1007/BF02143072

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  • DOI: https://doi.org/10.1007/BF02143072

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