Effects of β-endorphin on rat mast cells
- 10 Downloads
The endogenous opioid peptide β-endorphin induced a dose-dependent release of histamine from rat peritoneal mast cells. The threshold concentration was around 10−6M and the optimal concentration of 2×10−5M released 35% of the total histamine. The release of histamine was very rapid, complete within 10 s, and very similar to that induced by neurotensin or compound 40/80. The histamine release was temperature dependent and inhibited at increased extracellular calcium concentrations. Taken together, the findings indicate that the release of histamine induced by β-endorphin is a specific, energydependent process. The pattern of release has much in common with that induced by other basic peptides, such as neurotensin, dynorphin and substance P.
KeywordsPeptide Mast Cell Histamine Calcium Concentration Optimal Concentration
Unable to display preview. Download preview PDF.
- A. I. Faden and J. W. Holaday,Experimental endotoxin shock: The pathophysiologic function of endorphins and treatment with opiate antagonists, J. infectious Dis.142, 229–238 (1980).Google Scholar
- K. Nordlind and V. Mutt,Modulating effect of beta-endorphin, somatostatin, substance P and vasoactive intestinal peptide on the proliferative response of peripheral blood T lymphocytes of nickel-allergic patients to nickel sulfate, Int. Archs. Allergy appl. Immun.81, 368–370 (1986).Google Scholar
- H. Shibata, M. Mio and K. Tasaka,Analysis of the mechanism of histamine release induced by substance P, Biochim. Biophys, Acta846, 1–7 (1985).Google Scholar