Effects of β-endorphin on rat mast cells
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The endogenous opioid peptide β-endorphin induced a dose-dependent release of histamine from rat peritoneal mast cells. The threshold concentration was around 10−6M and the optimal concentration of 2×10−5M released 35% of the total histamine. The release of histamine was very rapid, complete within 10 s, and very similar to that induced by neurotensin or compound 40/80. The histamine release was temperature dependent and inhibited at increased extracellular calcium concentrations. Taken together, the findings indicate that the release of histamine induced by β-endorphin is a specific, energydependent process. The pattern of release has much in common with that induced by other basic peptides, such as neurotensin, dynorphin and substance P.
KeywordsPeptide Mast Cell Histamine Calcium Concentration Optimal Concentration
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