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The effect of phenazine methosulfate on the activity of dehydrogenases as studied by starch gel electrophoresis

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Résumé

L'effet de la phenazine methosulfate (PMS) sur l'activité de plusieurs enzymes oxydatifs a été étudié. En tant que transporteur d'électron, la PMS a un rôle important sur la coloration des isoenzymes séparés par électrophorèse en gel d'amidon. En ce qui concerne la déshydrogénase lactique, un excès de PMS provoque une coloration diffuse, alors que dans le cas de la déshydrogénase succinique on observe l'apparition de «bandes négatives» qui n'ont pas encore été décrites.

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Authors and Affiliations

  1. Yerkes Regional Primate Research Center, Emory University, 30322, Atlanta, Georgia, USA

    S. L. Manocha, G. H. Bourne & M. Sandler

Authors
  1. S. L. Manocha
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  2. G. H. Bourne
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  3. M. Sandler
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Additional information

This work was supported by grants Nos. HEO4553, HeO45691, and FRO0165 of the National Institute of Health. The technical assistance of Mr. R.Hernandez is gratefully acknowledged.

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Manocha, S.L., Bourne, G.H. & Sandler, M. The effect of phenazine methosulfate on the activity of dehydrogenases as studied by starch gel electrophoresis. Experientia 23, 35–36 (1967). https://doi.org/10.1007/BF02142253

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