Plant and Soil

, Volume 80, Issue 3, pp 337–344 | Cite as

Saprophytic competence of acid tolerant strains ofRhizobium trifolii in acid soil

  • Frank C. Thornton
  • C. B. Davey


Laboratory prescreening ofRhizobium trifolii for acid tolerance, based upon the ability of rhizobia to grow in acid media (pH 4.2) containing Al (15 μM), was successful for the selection of strains capable of survival in acid soil.

Both sterile and non-sterile soils of varying acidity were inoculated with several strains ofR. trifolii.

Acid tolerant strains generally had significantly higher populations at every sample period than an acid sensitive strain. Amelioration of soil acidity by liming improved persistence of all strains. Soil sterilization by autoclaving adversely affected survival of all strains at each soil acidity level.

Key words

Acid tolerance Persistence Rhizobium trifolii 


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  1. 1.
    Baver L D, Gardner W H and Gardner W R 1972 Soil Physics. 4th Ed. John Wiley and Sons Inc., New York. 498 p.Google Scholar
  2. 2.
    Brockwell J 1963 Accuracy of a plant-infection-technique for counting populations ofRhizobium trifolii. Appl. Microbiol. 11, 377–383.Google Scholar
  3. 3.
    Brockwell J, Bryant W G and Gault R R 1972 Persistence ofRhizobium trifolii in association with white clover at high elevations. Aust. J. Expt. Agric. Anim. Husb. 12, 407–413.CrossRefGoogle Scholar
  4. 4.
    Bromfield E S P and Jones D G 1980 Studies on acid tolerance ofRhizobium trifolii in culture and soil. J. Appl. Bacteriol. 48, 253–264.Google Scholar
  5. 5.
    Chatel D L, Greenwood R M and Parker C A 1968 Saprophytic competence as an important character in the selection ofRhizobium for inoculation. Proc. Int. Congr. Soil Sci., 9th, 1968, Vol. 2, pp. 65–73.Google Scholar
  6. 6.
    Clark F E 1965 Agar-plate method for total microbial counts.In Black C A (ed.) Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties. Agronomy 9, 1460–1466. Am. Soc. Agron., Madison, Wisc.Google Scholar
  7. 7.
    Cochran W G 1950 Estimation of bacterial densities by means of the “Most Probable Number”. Biometrics 6, 105–116.PubMedGoogle Scholar
  8. 8.
    Danzo S K A and Alexander M 1974 Survival of two strains of Rhizobium in soil. Soil Sci. Soc. Am. Proc. 38, 86–89.Google Scholar
  9. 9.
    Jones D G 1966 The contribution of white clover to a mixed upland sward. II. Factors effecting the density and effectiveness ofRhizobium trifolii. Plant and Soil 24, 250–260.Google Scholar
  10. 10.
    Kamprath E J 1970 Exchangeable aluminium as a criterion for liming leached mineral soils. Soil Sci. Soc. Am. Proc. 34, 252–254.Google Scholar
  11. 11.
    Keyser H H and Munns D N 1979 Tolerance of rhizobia to acidity, aluminium, and phosphate. Soil Sci. Soc. Am. J. 43, 519–523.Google Scholar
  12. 12.
    Mahler R L and Wollum A G II 1980 Influence of water potential on the survival of rhizobia in a Goldsboro loamy sand. Soil Sci. Soc. Am. J. 44, 988–992.Google Scholar
  13. 13.
    McClure P R and Israel D W 1979 Transport of nitrogen in the xylem of soybean plants. Plant Physiol. 64, 411–416.Google Scholar
  14. 14.
    Mehlich A 1978 New extractant for soil test evaluation of P, K, Mg, Ca, Mn, Na and Zn. Comm. Soil Sci. Plant Anal. 9, 477–492.Google Scholar
  15. 15.
    Thornton F C and Davey C B 1983a Acid tolerance ofRhizobium trifolii in culture media. Soil Sci. Soc. Am. J. 47, 496–501.Google Scholar
  16. 16.
    Thornton F C and Davey C B 1983b Response of the clover-Rhizobium symbiosis to soil acidity and Rhizobium strain. Agron. J. 75, 557–560.Google Scholar
  17. 17.
    Vincent J 1970 A Manual for the Practical Study of the Root-Nodule Bacteria. IBP Handbook No. 15. Blackwell Sci. Publ., Oxford. 164 pp.Google Scholar
  18. 18.
    Vincent J and Waters L M 1954 The root-nodule bacteria as a factor in clover establishment in the red basaltic soils of the Lismore District, N.S. Wales. Aust. J. Agr. Res. 58, 61–76.CrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1984

Authors and Affiliations

  • Frank C. Thornton
    • 1
  • C. B. Davey
    • 2
  1. 1.Department of Biological SciencesUniversity of WaikatoHamiltonNew Zealand
  2. 2.Departments of Forestry and Soil ScienceNorth Carolina State UniversityRaleighUSA

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