Saprophytic competence of acid tolerant strains ofRhizobium trifolii in acid soil
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Laboratory prescreening ofRhizobium trifolii for acid tolerance, based upon the ability of rhizobia to grow in acid media (pH 4.2) containing Al (15 μM), was successful for the selection of strains capable of survival in acid soil.
Both sterile and non-sterile soils of varying acidity were inoculated with several strains ofR. trifolii.
Acid tolerant strains generally had significantly higher populations at every sample period than an acid sensitive strain. Amelioration of soil acidity by liming improved persistence of all strains. Soil sterilization by autoclaving adversely affected survival of all strains at each soil acidity level.
Key wordsAcid tolerance Persistence Rhizobium trifolii
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