Summary
The ability of bacteroids, isolated fromLupinus luteus L. nodules at the stage of active nitrogen fixation, to assimilate (1-14C)-glucose and (2-14C)-glucose was being studied. The label is incorporated into all the Krebs cycle metabolites, amino acids and sugars after 5 min of glucose insertion into cell suspension. High activity of glucose phosphorylation was found in bacteroidsin vitro, the reaction rate being the highest at a glucose concentration of over 100 mM.
In lupine nodules sugars can be essential carbon substrate delivered to the bacteroids from host-plant cells. This point of view is discussed.
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References
Anderson R L and Kamel M Y 1966 Metods in Enzymology. Wood W A, Ed., Academic Press, New York-London 9, 388–392.
Antoniw L D and Sprent J I 1978 Primary metabolites ofPhaseolus vulgaris nodules. Phytochemistry 17, 675–678.
Bach M K, Magree W E and Burris R H 1958 Translocation of photosynthetic products to soybean nodules and their role in nitrogen fixation. Plant Physiol. 33, 118–124.
Chermenskaya I E and Petrova A N 1974 Study of enzymes of carbohydrate metabolism in yellow lupin nodules. Prikl. Biochim. Microbiol. 10, 269–274 (In Russian).
Glenn A R and Dilworth M J 1981 The uptake and hydrolysis of disaccharides by fast- and slow-growing species of Rhizobium. Arch. Microbiol. 129, 233–240.
Glenn A R, McKay I, Arwas R and Dilworth M J 1984 Sugar metabolism and the symbiotic properties of carbohydrate mutants ofRhizobium leguminosarum. J. Gen. Microbiol. 130, 239–245.
Glenn A R, McKay I, Arwas R and Dilworth M J 1984 Advances in Nitrogen Fixation Research. Nijhoff M and Junk W, Publ., Hague, 255 p.
Hais I M and Macek K 1962 Paper chromatography. Inostr. Literatura, Moscow, 851 p.
Hardy R W F 1977 Rate-limiting steps in biological photoproductivity. Genetic Engineering for Nitrogen Fixation. Hollaender A. Plenum Press, London-New York, 9, 401–408.
Hudman J F and Glenn A R 1980 Glucose uptake by free living and bacteroid forms ofRhizobium leguminosarum. Arch. Microbiol. 128, 72–77.
Keele B B, Hamilton P B and Elkan G H 1969 Glucose catabolism inRhizobium japonicum. J. Bacteriol. 97, 1184–1191.
Reibach P H and Streeter J G 1983 Metabolism of14C-labelled photosynthate and distribution of enzymes of glucose metabolism in soybean nodules. Plant Physiol. 72, 634–640.
Rigaud J, Bergersen F J, Turner G L and Daniel R M 1973 Nitrate dependent anaerobic acetylene reduction and nitrogen fixation by soybean bacteroids. J. Gen. Microbiol. 77, 137–144.
Romanov V I, Abdullayeva B R, Ivanov R F and Kretovich W L 1984 CO2 fixation by bacteroids isolated from lupine nodules. Prikl. Biochim. Microbiol. 20, 401–403 (In Russian).
Romanov V I, Fedulova N G, Chermenskaya I E, Shramko V I, Molchanov M I and Kretovich W L 1980 Metabolism of poly-β-hydroxybutyric acid in bacteroids inRhizobium lupini in connection with nitrogen fixation and photosynthesis. Plant and Soil. 56, 379–390.
Ronson C W and Primrose S B 1979 Carbohydrate metabolism inRhizobium trifolii: identification and symbiotic properties of mutants. J. Gen. Microbiol. 112, 77–88.
Trinchant J C, Birot A M, Rigaud J 1981 Oxygen supply and energy-yielding substrates for nitrogen fixation (acetylene reduction) by bacteroid preparations. J. Gen. Microbiol. 125, 159–165.
Vries G E, Van Brussel A A N, Quispel A 1982 Mechanism and regulation of glucose transport inRhizobium leguminosarum. J. Bacteriol. 149, 872–879.
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Kretovich, W.L., Romanov, V.I., Abdullaeva, B.R. et al. 14C-glucose utilization byRhizobium lupini bacteroids. Plant Soil 85, 211–217 (1985). https://doi.org/10.1007/BF02139625
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DOI: https://doi.org/10.1007/BF02139625