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Experientia

, Volume 27, Issue 9, pp 1029–1030 | Cite as

Action de divers effecteurs sur l'activité biologique de la mannosyl-transférase microsomique des splénocytes de Rat

  • M. Richard
  • P. Broquet
  • P. Louisot
Specialia Chimica, Biochimica, Biophysica

Summary

The splenic cellular microsomic mannosyltransferase is inhibited by detergent (Triton X 100 and Cemulsol), deoxycholate, dithiotreitol and cyclcheximide, but activated by nucleoside-triphosphates (ATP and GTP) and analogous (β γ-methylene-ATP or GTP); the enzyme is also activated by puromycine. This is good evidence for the sensitivity of the enzyme to any alteration of subcellular architecture at the microsomal level, and for the possibility of good transglycosylation when polypeptide is detached of ribosomes.

Literatur

  1. 1.
    P. Louisot etR. Got, Bull. Soc. Chim. biol.52, 455 (1970).Google Scholar
  2. 2.
    R. Letoublon, M. Richard, P. Louisot etR. Got, Europ. J. Biochem.18, 194 (1971).Google Scholar
  3. 3.
    M. Richard, R. Letoublon, P. Louisot etR. Got, Biochim. biophys. Acta,230, 603 (1971).Google Scholar
  4. 4.
    J. F. Caccam, J. J. Jackson etE. H. Eylar, Biochem. Biophys. Res. Commun.35, 505 (1969).Google Scholar
  5. 5.
    J. Molnar, H. Chao etG. Markivic, Arch. Biochem. Biophys.134, 533 (1969).Google Scholar
  6. 6.
    J. Molnar, M. Tetas etH. Chao, Biochem. Biophys. Res. Commun.37, 684 (1969).Google Scholar
  7. 7.
    M. Richard, P. Broquet, R. Got etP. Louisot, Biochimie,53, 107 (1971).Google Scholar
  8. 8.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr etR. J. Randall, J. biol. Chem.193, 265 (1951).Google Scholar

Copyright information

© Birkäuser Verlag 1971

Authors and Affiliations

  • M. Richard
    • 1
    • 2
  • P. Broquet
    • 1
    • 2
  • P. Louisot
    • 1
    • 2
  1. 1.Université de Lyon, Unité Médicale d'EnseignementFrance
  2. 2.de Recherches Lyon-Sud, Laboratoire de BiochimieOullinsFrance

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