Summary
Detection of ribonucleases in polyacrylamide gels after disc-electrophoresis is possible by incubation of the gels in a solution of low molecular RNA followed by staining with methylene blue. Application of this method to protein extracts of wheat leaves, sugar beet leaves and roots of different bean varieties as well as callus cultures and roots of the same bean variety shows differences in number and position of RNase zones.
Similar content being viewed by others
Literatur
G. Riou undC. Paoletti, Nature199, 65 (1963).
M. Wagner, J. Chromat.15, 108 (1964).
C. Poort undW. J. W. van Venroy, Nature204, 684 (1964).
N. Ressler, E. Olivero, G. R. Thopson undR. R. Joseph, Nature210, 695 (1966).
J. Uriel undJanine Courcon, C. r. hebd. Acad. Sci., Paris253, 1876 (1961).
J. Boyd andH. K. Mitchell, Analyt. Biochem.13, 28 (1965).
R. Warick, in Vorbereitung.
B. J. Davis, Ann. N.Y. Acad. Sci.121, 404 (1964).
B. Lerch, Experientia24, 889 (1968).
H. R. Maurer,Disk Elektrophorese (W. de Gruyter & Co., Berlin 1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wolf, G. Nachweis pflanzlicher Ribonukleasen in Polyacrylamid-Gelen nach Disk-Elektrophorese. Experientia 24, 890–891 (1968). https://doi.org/10.1007/BF02138632
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02138632