Kinetik der Reaktion von Imidazol-SH-Verbindungen mit Ellman Reagenz [5,5′-Dithio-bis-(-2-nitrobenzoesäure)]
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The rate of reaction of some derivatives and peptides of cysteine and a number of imidazole-SH-compounds with 5,5′-Dithio-bis-(2-nitrobenzoic acid) (DTNB) as well as the dependence of the reaction rate upon pH were investigated; for several compounds an equation is given, by which the rate constants and half reaction times can be estimated for various pH values. For some of the imidazole-SH-compounds studied thepK of the SH-group is no criterion of their reactivity with DTNB.
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