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Experientia

, Volume 27, Issue 4, pp 449–451 | Cite as

Immunpräzipitation Insulin-verwandter proteine mit ziegen-anti-insulinserum

  • M. Ziegler
  • K. -D. Kohnert
  • U. Karg
Specialia Haematologica

Summary

Bovine insulin, proinsulin, and intermediate form were prepared by Sephadex gelfiltration and chromatography on DEAE-cellulose. Using a goat anti-insulin serum, the cross-reactions of proinsulin and intermediate form could be shown by immuno-precipitation. A method is described for the separation of proinsulin from insulin in agar gel. New compounds related to proinsulin or insulin were found in human insulin preparations by means of immunoelectrophoresis.

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Copyright information

© Birkhäuser Verlag 1971

Authors and Affiliations

  • M. Ziegler
    • 1
  • K. -D. Kohnert
    • 1
  • U. Karg
    • 1
  1. 1.Zentralinstitut für Diabetes « Gerhardt Katsch », Experimenteller BereichKarlsburgDDR

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