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The denaturation of proteins: Two state? Reversible or irreversible?

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Zusammenfassung

Untersuchungen der Denaturie-rungsvorgänge sind für ein Verständnis der Proteinstruktur und -funktion von grosser Bedeutung. Es werden Methoden besprochen, die am ehesten geeignet sind, die Fragen zu beleuchten, ob die Denaturierung einen Zwei-Formen-Prozess darstellt und ob sie reversibel oder irreversibel ist. Sie werden an Beispielen der Harnstoffdenaturierung genetischer Varianten des Rinder-β-Lactoglobulins erläutert. Es wird ausserdem erörtert, in wieweit sich die Schlussfolgerungen auf die Denaturierung anderer Proteine übertragen lassen.

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Author notes

  1. G. B. Ralston

    Present address: Chemical Department, Carlsberg Laboratorium, Copenhagen, Denmark

Authors and Affiliations

  1. Department of Physical Biochemistry, Institute of Advanced Studies, Australian National University, 2601, Canberra, A.C.T., Australia

    H. A. McKenzie & G. B. Ralston

Authors
  1. H. A. McKenzie
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  2. G. B. Ralston
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McKenzie, H.A., Ralston, G.B. The denaturation of proteins: Two state? Reversible or irreversible?. Experientia 27, 617–624 (1971). https://doi.org/10.1007/BF02136923

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