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The indigogenic reaction for histochemical demonstration of alkaline and acid phosphatase

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Résumé

Le principe indigogénique à la détection histochimique des phosphates alcalins et acides a été appliqué. Ces substrates ont l'avantage de permettre une localisation précise de l'enzyme, sans ou avec minime diffusion. C'est aussi une méthode simple et directe pour la mise en évidence de ces enzymes sans passer par une réaction couplée. Un bisindigo très insoluble a lieu apparaît aux points d'activité enzymatique. Le 5-bromo-4-chlorocomposé donne un indigo bleu-vert tandis que le 5-bromo-6-chloro-composé donne un indigo magenta.

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Authors and Affiliations

  1. Department of Pathology, Wayne State University School of Medicine and the Detroit Institute of Cancer Research, Detroit, Michigan, USA

    P. L. Wolf, J. P. Horwitz, J. Vazquez, J. Chua, M. S. Y. Pak & Elisabeth von der Muehll

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  1. P. L. Wolf
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  2. J. P. Horwitz
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  3. J. Vazquez
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  4. J. Chua
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  5. M. S. Y. Pak
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  6. Elisabeth von der Muehll
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Wolf, P.L., Horwitz, J.P., Vazquez, J. et al. The indigogenic reaction for histochemical demonstration of alkaline and acid phosphatase. Experientia 23, 183–185 (1967). https://doi.org/10.1007/BF02136274

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