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Effect of organic solvent concentration on radioimmunoassay of aflatoxin B1

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Abstract

The effect of acetone and methanol contents in an aqueous casein-buffer solution pipetted with aflatoxin B1 into radioimmunoassay procedure, on some parameters of radioimmunochemical determination of aflatoxin B1 has been studied. These organic solvents lower an antiserum titer and value of the zero specific binding, and at higher concentrations make worse the detection limit and the accuracy of radioimmunoassay. However, in radioimmunoassay of food extracts containing subresidual levels of aflatoxin, it could be advantageous to add the extract volume to the organic solvent concentration of 60%.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28, Prague 6, Czechoslovakia

    L. Fukal

  2. Department of Veterinary Medicine, University of Agriculture, 165 21, Prague 6, Czechoslovakia

    H. Reisnerová

Authors
  1. L. Fukal
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  2. H. Reisnerová
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Fukal, L., Reisnerová, H. Effect of organic solvent concentration on radioimmunoassay of aflatoxin B1 . Journal of Radioanalytical and Nuclear Chemistry, Articles 132, 315–319 (1989). https://doi.org/10.1007/BF02136090

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  • DOI: https://doi.org/10.1007/BF02136090

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