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Electrophoretic mobilities and levels of T and B lymphocytes in human pregnancy

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Summary

Automatic cell electrophoresis has been used to investigate electrokinetic properties and the levels of T and B lymphocytes in peripheral blood of 124 pregnant women at different stages of gestation, and in 44 healthy controls. The electrophoretic mobilities (EPM) of T and B lymphocytes in maternal peripheral blood vary only slightly throughout pregnancy. In the pregnant subjects, there is a small but significant reduction in the percentage of circulating T lymphocytes. It is concluded that an increased electrostatic repulsion between maternal lymphocytes and trophoblast cells by alterations in the surface charge of lymphocytes plays an unimportant role in protecting the allogenic fetus from maternal immune attack.

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References

  1. Baines MG, Pross HF, Millar KG (1977) Lymphocyte populations in peripheral blood during normal human pregnancy. Clin Exp Immunol 28: 453–457

    Google Scholar 

  2. Birkeland SA, Kristoffersen K (1980) A longitudinal study of B lymphocyte responses during normal human pregnancy. J Reprod Immunol 2: 23–27

    Google Scholar 

  3. Bóyum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest (Suppl 97) 21: 28–29

    Google Scholar 

  4. Brain P, Marston RH, Gordon J (1972) Immunological responses in pregnancy. Br Med J 25: 488

    Google Scholar 

  5. Bulmer R, Hancock KW (1977) Depletion of circulating T lymphocytes in pregnancy. Clin Exp Immunol 28: 302–305

    Google Scholar 

  6. Campion PD, Currey HLF (1972) Cell-mediated immunity in pregnancy. Lancet 2: 830

    Google Scholar 

  7. Chassagne J, Chollet Ph, Ferriere JP, Touzet Cl, Ruse-Riol F, Plagne R (1981) “Null” and T lymphocyte subsets measured by manual electrophoresis during BCG immunotherapy of cancer patients. In: Preece AW, Light PA (eds) Cell electrophoresis in cancer and other clinical research. Elsevier/North-Holland Biomedical Press, Amsterdam New York Oxford, p 225

    Google Scholar 

  8. Chollet Ph, Chassagne J, Thierry C, Sauvezie B, Serrou B, Plagne R (1977) Isolation and electrophoretic mobility of three lymphoid populations in normal human blood. Eur J Cancer 13: 333–339

    Google Scholar 

  9. Dodson MG, Kerman RH, Lange CF, Stefani SS, O'Leary JA (1977) T and B cells in pregnancy. Obstet Gynecol 49: 299–302

    Google Scholar 

  10. Faulk WP (1980) Immunology of the maternofoetal relationship. In: Fougereau M, Dausset J (eds) Immunology, vol 80. Academic Press, New York San Francisco London, p 1093

    Google Scholar 

  11. Hanjan SNS, Talwar GP, Kidwai Z, Nath J (1977) Delineation and quantitation of human peripheral blood lymphocyte subpopulations by electrophoretic mobility and role of surface charge in cell to cell interaction. J Immunol 118: 235–241

    Google Scholar 

  12. Hirahara F, Gorai J, Tanaka K, Matsuzaki Y, Sumiyoshi Y, Shiojima Y (1980) Thymic hormonal activity on human peripheral blood lymphocytes in-vitro. Clin Exp Immunol 41: 353–359

    Google Scholar 

  13. Kaplan ME, Woodson M, Clark C (1976) Identification of T and B cell subpopulations in human peripheral blood: Electrophoretic mobility distributions associated with surface markers characteristics. J Immunol 117: 115–123

    Google Scholar 

  14. Knippel E, Kraskina N, Bliacher M, Fedorowa J (1984) Electrophoretic characterization of specific antibody effects on lymphocytes. Folia Biol (Praha) (in press)

    Google Scholar 

  15. Knippel E, Sabolovic D, Thomaneck U, Rychly J, Schütt W (1984) Scand J Immunol (in press)

  16. Knoblock V, Jouja V, Svoboda M (1975) Morphological changes in maternal lymphocytes in pregnancy. Br J Obstet Gynaecol 82: 146–150

    Google Scholar 

  17. Löffler H (1961) Cytochemischer Nachweis unspezifischer Esterase in Ausstrichen. Klin Wochenschr 39: 1220–1227

    Google Scholar 

  18. Loke YW, Brook SS, Allen GE (1975) Increase in IgM-bearing lymphocytes in peripheral blood of pregnant women. Am J Obstet Gynecol 122: 561–564

    Google Scholar 

  19. Loke YW (1978) Immunology and immunopathology of the human foetomaternal interaction. Elsevier, North Holland, p 27

    Google Scholar 

  20. Loke YW, Cater DB, Whyte A, Brook SS, Day S (1980) Electrophoretic mobilities of human trophoblast cells and lymphocytes from pregnant women. J Reprod Immunol 2: 45–52

    Google Scholar 

  21. Nordling S, Anderson LC, Hayry P (1972) Separation of B and T lymphocytes by preparative cell electrophoresis. Eur J Immunol 2: 405–410

    Google Scholar 

  22. Pohl A, Schütt W (1978) Increased reliability and productivity of cell electrophoresis by objectivated and automated measurements and data processing. In: Modern trends in cell electrophoresis. Medical Academy, Dresden, pp 92–107

    Google Scholar 

  23. Pretlow TG, Pretlow TP (1979) Cell electrophoresis. Int Rev Cytol 61: 85–128

    Google Scholar 

  24. Rocklin RE, Kitzmiller JL, Kaye MD (1979) Immunobiology of the maternal-fetal relationship. Annu Rev Med 30: 375

    Google Scholar 

  25. Rychly J, Babusikova O, Konikova E, Anders O (1984) Cell electrophoretic characterization of peripheral blood lymphocyte subpopulations from normal induviduals an CLL patients after enrichment by rosette formation. Neoplasma 31: 57–60

    Google Scholar 

  26. Sabolović D, Sabolović N, Dumont F (1972) Identification of T and B cells in mouse and man. Lancet 2: 927

    Google Scholar 

  27. Sato A, Sekija S (1926) A simple method for differentiation of myeloid and lymphatic leucocytes of the human blood. Tohoku J Exp Med 7: 111–115

    Google Scholar 

  28. Schütt W, Thomaneck U, Knippel E, Rychly J (1983) Applications of an automated cell electrophoresis equipment with high resolution — an overview. In: Hirai H (ed) Electrophoresis '83. International Conference on Electrophoresis and Related Fields, May 9–12, 1983, Walter de Gruyter & Co., Berlin New York

    Google Scholar 

  29. Straube W, Hofmann R, Klausch B (1979) Schwangerschaftsimmunologie. Neuere Aspekte der immunologischen Mutter-Kind-Beziehungen. Zentralbl Gynakol 101: 1169–1186

    Google Scholar 

  30. Strelkauskas AJ, Wilson BS, Dray S (1975) Inversion of levels of human T and B cells in early pregnancy. Nature 258: 331–332

    Google Scholar 

  31. Strelkauskas AJ, Davies IJ, Dray S (1978) Longitudinal studies showing alterations in the levels and functional response of T and B lymphocytes in human pregnancy. Clin Exp Immunol 32: 531–539

    Google Scholar 

  32. Vanderbeeken Y, Vlieghe MP, Delespesse G, Duchateau J (1982) Characterisation of immunoregulatory T cells during pregnancy by monoclonal antibodies. Clin Exp Immunol 48: 118–120

    Google Scholar 

  33. Waltzer WC, Baker DA, Pullis CK, Bachvaroff RJ, Rapoport FT (1982) Alterations in lymphocyte subpopulations during human pregnancy. Transplantation 34: 307–308

    Google Scholar 

  34. Wiig JN, Thundt S (1973) Electrophoresis of lymphoid cells: characterization of human B and T cells in peripheral lymphoid tissues. Clin Exp Immunol 15: 497–506

    Google Scholar 

  35. Wioland M, Sabdović D, Burg C (1972) Electrophoretic mobilities of T and B cells. Nature 237: 274–276

    Google Scholar 

  36. Wrezlewicz W, Rashkoff E, Lucas T, Kamasamy N, Sawyer PN (1977) Effects of human chorionic gonadotropin and anti-thymocyle globulin on the electrophoretic mobility of human lymphocytes. Transplant Proc 9: 1441–1446

    Google Scholar 

  37. Zeiller K, Hannig K, Pascher G (1971) Free flow electrophoretic separation of lymphocytes. Separation of graft versus host reactive lymphocytes. Hoppe-Seylers Z Physiol Chem 352: 1168–1170

    Google Scholar 

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Authors and Affiliations

  1. Klinik für Innere Medizin, Wilhelm-Pieck-Universität Rostock, Rostock, German Democratic Republic

    E. Knippel, W. Schütt & E. Behm

  2. Klinik für Geburtshilfe und Gynäkologie, Wilhelm-Pieck-Universität Rostock, Rostock, German Democratic Republic

    W. Straube & V. Briese

  3. Department of Obstetrics and Gynaecology, Queen Mary's Hospital, Roehampton, London, England

    W. L. Whitehouse

Authors
  1. E. Knippel
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  2. W. Straube
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  3. W. Schütt
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  4. E. Behm
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  5. V. Briese
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  6. W. L. Whitehouse
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Knippel, E., Straube, W., Schütt, W. et al. Electrophoretic mobilities and levels of T and B lymphocytes in human pregnancy. Arch. Gynecol. 236, 61–67 (1984). https://doi.org/10.1007/BF02134000

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  • DOI: https://doi.org/10.1007/BF02134000

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