Skip to main content
SpringerLink
Log in

Influence of dextran macromolecules on the glycosaminoglycan metabolism of cultured corneal stroma and keratoconus fibroblast

  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

The addition of dextran macromolecules to the incubation medium at 1% and 10% concentration does not cause any alteration of sulphated glycosaminoglycan synthesis or its distribution within the various cell compartments. This is in contrast to observations on a serum-free and dextran-enriched medium in which glycosaminoglycan distribution of stored corneal tissue remains normal and the concentration of keratan sulphate is maintained. The mechanism of this action is unknown. Some experimental findings of the influence of dextran upon cell metabolism are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bleckmann H, Kresse H (1980) Glycosaminoglycan metabolism of cultured cornea cells derived from bovine and human stroma and from bovine epithelium. Exp Eye Res 30:469–479

    Article  PubMed  Google Scholar 

  • Dahl JM, Cöster L (1978) Proteoglycan biosynthesis in cultures of cornea and corneal stroma cells from adult rabbits. Exp Eye Res 27:175–190

    Article  PubMed  Google Scholar 

  • Fratantoni JC, Hall CW, Neufeld FF (1968) The defect in Hurler's and Hunter's syndrome: Foulty degradation of mucopolysacchide. Proc Nat Acad Sci USA 60:699–706

    PubMed  Google Scholar 

  • Funderburgh JL, Chandler JW (1980) Corneal keratan sulfate synthesis in vitro. Arvo 1980, Suppl Invest Ophthalmol Vis Sci p l47

  • Gamse G, Fromme HG, Kresse H (1978) Metabolism of sulfated glycosaminoglycans in cultured endothelial cells and smooth muscle cells from bovine aorta, Biochem Biophys Acta 544:514–528

    PubMed  Google Scholar 

  • Kresse H, von Figura K, Buddecke E, Fromme HG (1975) Metabolism of sulfated glycosaminoglycans in cultivated bovine arterial cells. 1. Characterization of different pools of sulfated glycosamino-glycans. Hoppe-Seyler's Z Physiol Chem 356:929–941

    PubMed  Google Scholar 

  • Madden PW, Easty DL (1982) Assessment and interpretation of corneal endothelial cell morphology and function following cryopreservation. Br J Ophthalmol 66:136–140

    PubMed  Google Scholar 

  • Meyer FA, Koblentz M, Silbergerg A (1977) Structural Investigation of loose connective tissue by using a series of dextran fractions and non-interacting macromolecules probes. Biochem J 161:285–291

    PubMed  Google Scholar 

  • Pearce RH, Laurent TC (1977) Exclusion of dextrans by meshworks of collagenous fibres. Biochem J 163:617–625

    PubMed  Google Scholar 

  • Peyman GA, Palacio M, Sanders DR (1980) Effect of dextran on corneas perfused at an increased pressure. Can J Ophthalmol 15:81–83

    PubMed  Google Scholar 

  • Pittz EP, Jones R, Golberg L, Caulston F (1977) Interaction of polysaccharides with plasma membranes. 1. Interaction of human erythrocytes with degraded iota carrageenans and the effect of dextran and DEAE dextran. Biorheology 14:21–31

    PubMed  Google Scholar 

  • Shively JE, Conrad MW (1976) Formation of anhydro sugars in the chemical depolymerization of heparin. Biochemistry 15:3932–3934

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universitätsaugenklinik Charlottenburg, Freie Universität-Berlin, D-Spandauer Damm 130, 1000, Berlin 19

    H. Bleckmann

Authors
  1. H. Bleckmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Prof. Dr. E. Buddecke on the occasion of his 60th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bleckmann, H. Influence of dextran macromolecules on the glycosaminoglycan metabolism of cultured corneal stroma and keratoconus fibroblast. Graefe's Arch Clin Exp Ophthalmol 221, 70–72 (1983). https://doi.org/10.1007/BF02133809

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02133809

Keywords

Search

Navigation