Archives of gynecology

, Volume 233, Issue 4, pp 229–240 | Cite as

Radioimmunoassay of 5α-Pregnane-3,20-Dione

A metabolite of placental progesterone
  • M. Zwirner
  • M. M. Fawzy
  • F. C. Bopp
  • E. Klemm-Wolfgram
  • D. Handschuh
  • W. Voelter
  • A. E. Schindler
Original Papers

Summary

A radioimmunoassay (RIA) procedure has been developed for the measurement of 5α-pregnane-3,20-dione (DHP) in human plasma after ether extraction of the plasma-samples, followed by column chromatography. The antiserum was generated in rabbits with 6α-carboxyethylmer-captoprogesterone-BSA conjugate. The high affinity (Ka = 3.72 × 109 1/mol) antiserum binds 40% of 40 picograms of tritiated 5α-pregnane-3,20-dione at working dilutions of 1 : 2800. Negligible cross-reactivity of the antiserum was detected with 5β-pregnane-3,20-dione (1.8%). Other hydroxylated preg-nanes showed minor cross-reactivity (5–40%). The cross-reacting steroids were all separated from pregnanedione by one chromatographic step. The plasma levels of 5α-pregnane-3,20-dione were measured from 22nd to 42nd week of normal pregnancy and compared to published data.

Key words

Pregnancy 5α-Pregnane-3,20-dione Radioimmunoassay 

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Copyright information

© J. F. Bergmann Verlag GmbH & Co KG 1983

Authors and Affiliations

  • M. Zwirner
    • 1
  • M. M. Fawzy
    • 1
  • F. C. Bopp
    • 1
  • E. Klemm-Wolfgram
    • 1
  • D. Handschuh
    • 1
  • W. Voelter
    • 2
  • A. E. Schindler
    • 1
  1. 1.Dept. of Obstetrics and GynecologyUniversity of TübingenTübingenFederal Republic of Germany
  2. 2.Dept. of Organic and Physical BiochemistryUniversity of TübingenTübingenFederal Republic of Germany

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