Abstract
A variety of mechanical (intensity of the light source, the width and the angle of the exciting source) and physiological factors (retinal pigmentation, vitreous fluorescence) and many systemic factors affect the results of vitreous fluorophotometry. These factors are discussed with respect to identifying those that are inconsequential and those that are significant. Methods to compensate for the important factors are described.
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References
Andersson L-O, Rehnström A, Eaker DL (1971) Studies on “non-specific” binding: the nature of the binding of fluorescein to bovine serum albumin. Eur J Biochem 20:371–380
Boettner EA, Wolter JR (1962) Transmission of the ocular media. Invest Ophthalmol 1:776–783
Brochner-Mortensen J, Ditzel J, Mogensen CE, Rodbro P (1979) Microvascular permeability to albumin and glomerular filtration rate in diabetic and normal children. Diabetologia 16:307–311
Cunha-vaz J, Maurice D (1969) Fluorescein dynamics in the eye. Doc Ophthalmol 26:61–72
Delori FC, Castany MA, Webb RH (1978) Fluorescence characteristics of sodium fluorescence in plasma and whole blood. Exp Eye Res 27:417–425
Dolhofer R, Weiland OH (1979) Increased glycosylation of serum albumin in diabetes mellitus. Diabetes 29:417–422
Guthrow CE, Morris MA, Day, JF, Thorpe SR, Baynes JW (1979) Enhanced nonenzymatic glucosylation of human serum albumin in diabetes mellitus. Proc Natl Acad Sci 76:4258–4261
Laurence DJR (1952) A study of the adsorption of dyes on bovine serum albumin by the method of polarization of fluorescence. Biochem J 51:168–180
McFarland KF, Catalano EW, Day JF, Thorpe SR, Baynes JW (1979) Nonenzymatic glucosylation of serum proteins in diabetes mellitus. Diabetes 28:1011–1014
Nagataki S (1975) Aqueous humor dynamics of human eyes as studied using fluorescein. Jpn J Ophthalmol 19:235–249
Sawa M, Araie M, Nagataki S (1981) Permeability of the human corneal endothelium to fluorescein. Jpn J Ophthalmol 25:60–68
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Mosier, M.A. Factors affecting vitreous fluorescence. Graefe's Arch Clin Exp Ophthalmol 222, 202–205 (1985). https://doi.org/10.1007/BF02133674
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DOI: https://doi.org/10.1007/BF02133674