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Laserthermia kills acidotic cancer cells more effectively than conventional hyperthermia

Abstract

The aim of this study was to investigate how acidosis affects the sensitivity of F9 embryonal carcinoma cells to conventionally induced hyperthermia and Nd-YAG laser-induced laserthermia. Nigericin, a polyether ionophore, was used to reduce the intracellular pH to 6.8, while the control cells were maintained at a physiological pH of 7.4. Acidosis increased the heat sensitivity significantly. After the water bath only 75% of the acidotic F9S1 cells were viable, whereas all of the control cells at pH 7.4 survived. When the laser was used, the difference was even more pronounced. Only 9% of the acidotic cells were viable after a 2-min treatment and 0.2% after a 4-min treatment. At physiological pH a 2-min treatment left 41% of the cells viable and a 4-min treatment 2%. This study showed that laserthermia is significantly more effective in killing cells with low intracellular pH than it is in killing cells with normal intracellular pH. The core of a tumour is often acidotic and thus radioresistant. It is suggested that laserthermia could be a good choice when treating acidotic radioresistant cancer cells, and its effect as an adjuvant to radiation therapy should be investigated.

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Castrén-Persons, M. Laserthermia kills acidotic cancer cells more effectively than conventional hyperthermia. Laser Med Sci 10, 31–36 (1995). https://doi.org/10.1007/BF02133161

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  • DOI: https://doi.org/10.1007/BF02133161

Key words

  • Embryonal carcinoma
  • F9 cells
  • Laser
  • Hyperthermia
  • Nigericin
  • Acidosis