Lasers in Medical Science

, Volume 10, Issue 1, pp 19–24 | Cite as

The Calibration of fibre optic probes used for light dosimetry measurements during photodynamic therapy

  • M. R. Stringer
  • E. J. Hudson
  • M. A. Smith
Original Articles


We describe the calibration of fibre optic probes used to perform in vivo light dosimetry studies during the treatment of skin lesions by photodynamic therapy. Results from six individual detectors show that the linearity of the calibration and the calculated radiant energy fluence rate within a liquid phantom are independent of the type and sensitivity of the probe. The method of calibration is also shown to yield the optical interaction coefficients of the phantom.

Key words

Photodynamic therapy Light dosimetry Fibre optic probes 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kennedy JC, Pottier RH, Pross DC. Photodynamic therapy with endogenous protoporphyrin IX. Basic principles and present clinical experience.J Photochem Photobiol [B] 1990,6:143–8CrossRefGoogle Scholar
  2. 2.
    Wolf P, Rieger E, Kerl H. Topical photodynamic therapy with endogenous porphyrins after application of 5-aminolevulinic acid.J Am Acad Dermatol 1993,28:17–21PubMedGoogle Scholar
  3. 3.
    Cairnduff F, Stringer MR, Hudson EJ et al. Superficial photodynamic therapy with topical 5-aminolaevulinic acid for superficial primary and secondary skin cancer.Br J Cancer 1994,69:605–8PubMedGoogle Scholar
  4. 4.
    Feather JW, King PR, Driver I, Dawson JB. A method for the construction of disposable cylindrical diffusing fibre optic tips for use in photodynamic therapy.Lasers Med Sci 1989,4:229–35CrossRefGoogle Scholar
  5. 5.
    Henderson B. An isotropic dosimetry probe for monitoring laser light in tissue. Theoretical and experimental assessment. PhD Thesis, Heriot Watt University, Medical Laser Unit, 1990Google Scholar
  6. 6.
    Hudson EJ, Stringer MR, Smith MA. Developments in the construction of radio-opaque, isotropic, fibre optic probes for light dosimetry studies in photodynamic therapy.Phys Med Biol 1993,38:1529–36CrossRefGoogle Scholar
  7. 7.
    Wilson BC, Patterson MS, Burns DM. Effect of photosensitizer concentration in tissue on the penetration depth of photoactivating light.Lasers Med Sci 1986,1:235–44CrossRefGoogle Scholar
  8. 8.
    Flock ST, Wilson BC, Patterson MS. Total attenuation coefficients and scattering phase function of tissues and phantom materials at 633 nm.Med Phys 1987,14:835–41CrossRefPubMedGoogle Scholar
  9. 9.
    Driver I, Feather JW, King P, Dawson JB. The optical properties of aqueous suspensions of Intralipid, a fat emulsion.Phys Med Biol 1989,34:1927–30CrossRefGoogle Scholar
  10. 10.
    Marijnissen JPA, Star WM. Quantitative light dosimetry in vitro and in vivo.Lasers Med Sci 1987,2:235–42Google Scholar
  11. 11.
    Withrow RB, Withrow AP.Radiation Biology Vol. III: Visible and Near Visible Light. New York: McGraw-Hill, 1956:196Google Scholar
  12. 12.
    Wilson BC, Patterson MS. The physics of photodynamic therapy.Phys Med Biol 1986,31:327–60CrossRefPubMedGoogle Scholar
  13. 13.
    Madsen SJ, Patterson MS, Wilson BC. The use of India ink as an optical absorber in tissue-simulating phantoms.Phys Med Biol 1992,37:985–93CrossRefPubMedGoogle Scholar
  14. 14.
    Hudson EJ, Stringer MR, Cairnduff F et al. The optical properties of skin tumours measured during superficial photodynamic therapy.Lasers Med Sci 1994,9:99–103Google Scholar

Copyright information

© W.B. Saunders Company Ltd 1995

Authors and Affiliations

  • M. R. Stringer
    • 1
  • E. J. Hudson
    • 1
  • M. A. Smith
    • 1
  1. 1.Research School of Medicine, Division of Clinical Science: Medical PhysicsUniversity of LeedsLeedsUK

Personalised recommendations