Mycopathologia et mycologia applicata

, Volume 37, Issue 4, pp 320–330 | Cite as

Flocculation phenomenon of Candida albicans by lysozyme

  • T. Kamaya
Article

Abstract

Young colonies of Sabouraud's glucose agar room temperature culture ofCandida species from human isolation were suspended in distilled water. The suspension was mixed with a solution of lysozyme and incubated in a 37° C water bath. Within 3–5 hours, various species ofCandida cells showed flocculation to varying degrees which occurred at varying periods of onset. Among sevenCandida species,Candida albicans andCandida stellatoidea showed the strongest flocculation, earliest onset and most solution clarity than did any other species.Candida stellatoidea was indistinguishable fromCandida albicans in its degree of flocculation, and in the clarity of solution.Candida species may be arranged in the following order according to their decreasing positivity in flocculation:
  1. 1

    Candida albicans

     
  2. 2

    Candida stellatoidea

     
  3. 3

    Candida tropicalis

     
  4. 4

    Candida krusei

     
  5. 5

    Candida pseudotropicalis

     
  6. 6

    Candida parapsilosis

     
  7. 7

    Candida guilliermondii

     
  8. 8

    Saccharomyces species may be placed afterCandida guilliermondii.

     

It seems possible to separate theCandida species into 3 groups by the rate of flocculation, and clarity of solution. Group I.Candida albicans andCandida stellatoidea. Group II.Candida tropicalis, C. krusei andCandida pseudotropicalis. Group III.Candida parapsilosis andCandida guilliermondii. Saccharomyces specimens (S. cerevisiae and others) were placed after group III.

Keywords

Candida Lysozyme Flocculation Candida Albicans Candida Tropicalis 

Zusammenfassung

Junge Kolonien vonCandida Arten, auf Sabouraud's Glucose Agar bei Zimmertemperatur, isoliert vom Menschen, wurden in destilliertem Wasser verteilt. Die Suspension wurde mit einer Lösung von Lysozym gemischt und bei 37° C im Wasserbad inkubiert. Innerhalb von 3–5 Stunden haben verschiedene Arten vonCandida-Zellen in verschiedenem Grade Flocculation gezeigt, deren Beginn zu verschiedenen Zeitpunkten erfolgte. Unter siebenCandida Arten zeigtenCandida albicans undCandida stellatoidea die stärkste Flocculation, den frühesten Beginn und größte Durchsichtigkeit der Lösung, denn irgendwelche andere Arten,C. stellatoidea konnte man vonC. albicans in der Rate der Flocculation und in dem Grade der Durchsichtigkeit der Lösung nicht unterscheiden.Candida Arten können in der folgenden Ordnung gemäß der abnehmenden Positivität eingereiht werden:C. albicans, C. stellatoidea, C. tropicalis, C. krusei, C. pseudotropicalis, C. parapsilosis, C. guilliermondii, Saccharomyces Arten. Es ist möglich, dieCandida Arten gemäß der Rate der Flocculation und der Durchsichtigkeit der Lösung in drei Gruppen einzuordnen. Gruppe 1:C. albicans andC. stellatoidea; Gruppe 2:C. tropicalis, C. krusei undC. pseudotropicalis; Gruppe 3:C. parapsilosis undC. guilliermondii. Saccharomyces Arten (S. cerevisiae und andere) wurden nach der Gruppe 3 eingeordnet.

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Copyright information

© Dr. W. Junk N.V. den Haag 1969

Authors and Affiliations

  • T. Kamaya
    • 1
  1. 1.Dept. of Clinical Pathology Deer Lodge HospitalD.V.A. WinnipegCanada

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