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Characterization of the 3′ untranslated region of the mouse homeobox geneHoxB5

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Abstract

The mouse pre-B cell line, 70Z/3, expresses multiple transcripts of the homeobox gene,HoxB5. We show here that this heterogeneity is due, at least in part, to the usage of alternative poly-A addition sites in the 3′ untranslated region (UT) of the primaryHoxB5 transcript. Furthermore, upon analysis of the subcellular distribution of the differentHoxB5 RNA species, we found that the transcripts are present mainly in the nucleus, with two-to-five-fold less RNA present in the cytoplasm. These studies suggest that multiple post-transcriptional regulatory mechanisms are involved in the expression ofHoxB5 RNA.

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Authors and Affiliations

  1. Department of Immunology, University of Toronto, M5S 1A8, Toronto, Canada

    C. C. K. Yu, C. J. Paige & G. E. Wu

  2. Wellesley Hospital Research Institute, M4Y 1J3, Toronto, Canada

    C. C. K. Yu, C. J. Paige & G. E. Wu

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  2. C. J. Paige
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Yu, C.C.K., Paige, C.J. & Wu, G.E. Characterization of the 3′ untranslated region of the mouse homeobox geneHoxB5. Experientia 51, 616–622 (1995). https://doi.org/10.1007/BF02128755

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