Abstract
The concentration-dependent effects of copper acting either as an antioxidant or as a prooxidant were examined in vitro using Tween 20-induced hemolysis. When cupric ion concentration was more than 10 μM, free copper(II) acted as a prooxidant; both extensive hemolysis and production of unknown thiobarbituric acid-reactive substance occurred in hamster and pig erythrocytes irrespective of vitamin E status. However, when cupric ion concentration was 2–4 μM in the incubation medium, copper showed a clear antioxidant activity, reducing both hemolysis and malondialdehyde production induced either by diluted peroxide-containing Tween 20 with ascorbic acid and sodium azide in vitamin E-deficient hamster erythrocytes, or by peroxide-containing Tween 20 in pig erythrocytes containing marginal amounts of vitamin E. Copper(II) is taken up by the erythrocytes, where copper(I)-complexes may contribute to the protection of cells with membrane vitamin E against oxidative radical attack.
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Moat, N. E., Evans, T. E., Quinlan, G. J., and Gutteridge, J. M. C., FEBS Lett.328 (1993) 103.
Gutteridge, J. M. C., Winyard, P. G., Blake, D. R., Lunec, J., Brailsford, S., and Halliwell, B., Biochem. J.230 (1985) 517.
Uauy, R., Castillo-Duran, C., Fisberg, M., Fernandez, N., and Valenzuela, A., J. Nutr.115 (1985) 1650.
Chahboun, S., Tallineau, C., Pontcharraud, R., Guettier, A., and Piriou, A., Biochim. biophys. Acta1042 (1990) 324.
Brigelius, R., Spöttl, R., Bors, W., Lengfelder, E., Saran, M., and Weser, U., FEBS Lett.47 (1974) 72.
Alberghina, M., Lupo, G., La Spina, G., Mangiameli, A., Gulisano, M., Sciotto, D., and Rizzarelli, E., J. inorg. Biochem.45 (1992) 245.
Olin, K. L., Walter, R. M., and Keen, C. L., Am. J. clin. Nutr.59 (1994) 654.
Balevska, P. S., Russanov, E. M., and Kassabova, T. A., Int. J. Biochem.13 (1981) 489.
Kato, N., Saari, J. T., and Schelkoph, G. M., J. Nutr. Biochem.5 (1994) 99.
Sherman, A. R., J. Nutr.122 (1992) 604.
Suttle, N. F., and Jones, D. G., Proc. Nutr. Soc.45 (1986) 317.
Braude, R., in: Copper in Animal Wastes and Sewage Sludge, p. 3. Eds P. L'Hermite and J. Dehandtschutter. D. Reidel Publishing Co., Dordrecht 1981.
Wendel, A., and Heidinger, S., Res. Commun. chem. Path. Pharmac.28 (1980) 473.
Schvartz, I., and Hazum, E., Biochem. biophys. Res. Commun.136 (1986) 417.
Hamada, T., and Matsumoto, M., Experientia36 (1980) 978.
Hamada, T., Furuya, M., and Hodate, K., Experientia38 (1982) 462.
Hamada, T., Hodate, K., and Nakayama, E., Experientia40 (1984) 258.
Hamada, T., Experientia50 (1994) 49.
Hamada, T., Maeda, S., Hodate, K., Nakayama, E., and Jimbu, M., Bull. natl Inst. Anim. Ind.43 (1985) 51.
Hansen, L. G., and Warwick, W. J., Clin. Biochem.3 (1970) 225.
Yasuda, H., Tamai, H., Miki, M., and Mino, M., J. nutr. Sci. Vitaminol.32 (1986) 245.
Azaz, E., Segal, R., and Milo-Goldzweig, I., Biochim. biophys. Acta646 (1981) 444.
Donbrow, M., Azaz, E., and Pillersdorf, A., J. pharm. Sci.67 (1978) 1676.
Mao, S. J. T., and Kottke, B. A., Biochim. biophys. Acta620 (1980) 447.
Simpson, J. A., Cheeseman, K. H., Smith, S. E., and Dean, R. T., Biochem. J.254 (1988) 519.
Fernandes, A., Mira, M. L., Azevedo, M. S., and Manso, C., Free Rad. Res. Commun.4 (1988) 291.
Smith, H. A., Dasmahapatra, A. K., Caffrey, J. M. Jr., and Frieden, E., Comp. Biochem. Physiol.91C (1988) 301.
Fernandes, A. C., Filipe, P. M., and Manso, C. F., Eur. J. Pharmac.220 (1992) 211.
Bush, J. A., Mahoney, J. P., Gubler, C. J., Cartwright, G. E., and Wintrobe, M. M., J. Lab. clin. Med.47 (1956) 898.
McArdle, H. J., Proc. Nutr. Soc.51 (1992) 199.
Massa, E. M., and Giulivi, C., Free Rad. Biol. Med.14 (1993) 559.
Milne, L., Nicotera, P., Orrenius, S., and Burkitt, M. J., Archs Biochem. Biophys.304 (1993) 102.
Ciriolo, M. R., Desideri, A., Paci, M., and Rotilio, G., J. biol. Chem.265 (1990) 11030.
Hartmann, H. J., Gärtner, A., and Weser, U., Clinica chim. Acta152 (1985) 95.
Crouch, R. K., Kensler, T. W., Oberley, L. W., and Sorenson, J. R. J., in: Biological and Inorganic Copper Chemistry, vol. 1, p. 139. Eds K. D. Karlin and J. Zubieta. Akademic Press, New York 1985.
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Hamada, T. Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E. Experientia 51, 572–576 (1995). https://doi.org/10.1007/BF02128746
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DOI: https://doi.org/10.1007/BF02128746