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Mycopathologia et mycologia applicata

, Volume 53, Issue 1–4, pp 183–200 | Cite as

The development and evaluation of immunodiffusion, immunofluorescence and physiological tests for the differentiation of atypical trichophyton rubrum and T. mentagrophytes isolates

  • Wallace E. Turner
  • William Kaplan
Article

Abstract

Five laboratory procedures: 1) immunodiffusion, 2) immunofluorescence, 3) in vitro hair perforation, 4) pigment stimulation, and 5) a urease test were compared for their ability to differentiateT. rubrum fromT. mentagrophytes. Of the physiological tests, thein vitro hair perforation technique was the most reliable for differentiating the two species. With the serological tests, the organisms were not differentiated by immunodiffusion, but if appropriate dilutions of the conjugates were used in immunofluorescence testing, most isolates could be differentiated.

Keywords

Perforation Serological Test Laboratory Procedure Physiological Test Trichophyton Rubrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ajello, L. & L. K. Georg (1957) In vitro hair cultures for differentiating between atypical isolates of Trichophyton mentagrophytes and Trichophyton rubrum. Mycopath. Mycol. Appl. 8: 3–17.CrossRefGoogle Scholar
  2. Ajello, L., L. K. Georg, W. Kaplan & L. Kaufman (1966) Laboratory Manual for Medical Mycology. U.S. Dept. HEW.Google Scholar
  3. Bocobo, F. C. & R. W. Benham (1949) Pigment production in the differentiation of Trichophyton mentagrophytes and Trichophyton rubrum. Mycologia 41: 291–302.Google Scholar
  4. Coons, A. H. & M. H. Kaplan (1950) Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. J. Exper. Med. 91: 1–13.CrossRefGoogle Scholar
  5. Dyson, J. E. & M. E. Landay (1963) Differentiation of Trichophyton rubrum from Trichophyton mentagrophytes. Mycopath. Mycol. Appl. 20: 81–97.CrossRefGoogle Scholar
  6. Goldman, M. (1957) Staining Toxoplasma gondii with fluorescein-labeled antibody. II A new serologic test for antibodies to Toxoplasma based upon inhibition of specific staining. J. Exper. Med. 105: 557–573.CrossRefGoogle Scholar
  7. Kaplan, W. & M. S. Ivens (1960) Fluorescent antibody staining of Sporotrichum schenckii in cultures and clinical materials. J. Invest. Dermat. 35: (3) 151–159.Google Scholar
  8. Landay, M. E. (1961) A morphological, physiological, and serological study of Trichophyton rubrum and Trichophyton mentagrophytes. M. S. Thesis, West Virginia University, Morgantown, West Virginia.Google Scholar
  9. Landay, M. E., R. W. Wheat, N. F. Conant & E. P. Lowe (1967) Serological comparison of the three morphological phases of Coccidioides immitis by the agar gel diffusion method. J. Bact. 93: 1–6.CrossRefPubMedGoogle Scholar
  10. Miura, T. & T. Kasai (1967) Immunofluorescent studies on Trichophyton asteroides compared with Trichophyton interdigitale. Tohoku J. Exp. Med. 93: 49–55.PubMedGoogle Scholar
  11. Philpot, C. (1967) The differentiation of Trichophyton mentagrophytes from T. rubrum by a simple urease test. Sabouraudia 5 (3): 189–193.PubMedGoogle Scholar
  12. Pinetti, P. & A. Lostia (1966) L'infezione dermatofitica del pelo in vitro. Rassegua Medica Sarda Ed. Collana di Monografie N. 13.Google Scholar
  13. Richards, O. W. & P. A. Waters (1967) A new interference exciter filter for fluorescence microscopy of fluorescein tagged substances. Stain. Tech. 42: 320–321.Google Scholar

Copyright information

© Dr. W. Junk B. V. den Haag 1974

Authors and Affiliations

  • Wallace E. Turner
    • 1
  • William Kaplan
    • 2
  1. 1.Department of Parasitology and Laboratory Practice, School of Public HealthUniversity of North CarolinaChapel Hill
  2. 2.Center for Disease Control, Health Services and Mental Health Administration, Public Health Service, U.S. Department of Health, Education, and WelfareAtlanta

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