Abstract
A specific and sensitive solid-phase radioimmunoassay has been developed for the detection of LCM virus antigens and anti-LCM virus antibodies. The test was performed in a microtiter system using polyvinylchloride wells coated with anti-LCM virus rabbit hyperimmune serum. LCM virus antigens were allowed to bind to this antibody and afterwards detected by125J-labeled anti-LCM virus-γ-globulin. Anti-LCM virus antibodies were assayed by specific inhibition of these bound antigens. Since this technique is rapid and easy to perform the solid-phase radioimmunoassay is a valuable test for detecting LCM virus infections. Selected at random, 208 girls with, and 208 girls without, contact to Syrian hamsters were investigated for anti-LCM virus antibodies. Five (2.4%) of the hamster-exposed girls had anti-LCM virus antibodies (RIA-titers 1∶8-1∶32) in contrast to none of the control group (P=0.03).
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Blechschmidt, M., Gerlich, W. & Thomssen, R. Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to Syrian hamsters. Med Microbiol Immunol 163, 67–76 (1977). https://doi.org/10.1007/BF02126711
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DOI: https://doi.org/10.1007/BF02126711