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Glucocorticoid receptors in alveolar macrophages: Methodological aspects of the determination of the number of glucocorticoid receptors per cell

  • Mediators of Hypersensitivity Reactions
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Abstract

Among patients with asthma or chronic obstructive pulmonary diseases the response to glucocorticoids varies considerably. To investigate the possible relationship between clinical response and number of glucocorticoid receptors in alveolar macrophages or theirK D value a micro receptor assay was developed. Assay conditions were adjusted because of receptor occupancy by endogenous or therapeutically used glucocorticoids and high aspecific binding.

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Authors and Affiliations

  1. Department of Pulmonary Medicine, Erasmus University and Academic Hospital Dijkzigt, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    P. Th. W. van Hal, H. C. Hoogsteden & C. Hilvering

  2. Department of Immunology, Erasmus University and Academic Hospital Dijkzigt, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    P. Th. W. van Hal & R. Benner

  3. Department of Biochemistry, Erasmus University and Academic Hospital Dijkzigt, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    E. Mulder

Authors
  1. P. Th. W. van Hal
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  2. E. Mulder
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  3. H. C. Hoogsteden
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  4. C. Hilvering
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  5. R. Benner
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van Hal, P.T.W., Mulder, E., Hoogsteden, H.C. et al. Glucocorticoid receptors in alveolar macrophages: Methodological aspects of the determination of the number of glucocorticoid receptors per cell. Agents and Actions 26, 128–131 (1989). https://doi.org/10.1007/BF02126584

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  • DOI: https://doi.org/10.1007/BF02126584

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